FastQCFastQC Report
Mon 6 Feb 2017
SRR2078215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842583
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20890.1133734545472307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.043.9800451
GTATCAA58050.040.933011
ATCAACG81150.029.3086433
TCAACGC82850.028.7909554
TATCAAC83050.028.6404692
CAACGCA85300.028.1265985
AACGCAG88700.026.93126
CTCGTAT1801.2732926E-1124.9032139
TTAGTCG1452.0281732E-823.78025239
ACGCAGA101500.023.1744147
CGCAGAG103500.022.6994468
TCGTATG2450.022.51844440
AGAGTAC103000.021.90469611
GAGTACT70500.021.72594812
TAGTCGG1756.2464096E-921.67400440
GCAGAGT109550.021.3490549
CAGAGTA107650.021.31102410
AGTACTT73650.020.9840913
GTACTTT73550.020.684314
TCTCGTA2201.9099389E-1020.37535538
Produced by FastQC (version 0.11.5)