FastQCFastQC Report
Mon 6 Feb 2017
SRR2078214_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078214_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755967
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.039.840211
GTATCAA35500.035.937941
TCAACGC44500.027.8883744
ATCAACG44650.027.8710983
TATCAAC46250.027.5016162
CAACGCA45500.027.1996785
AACGCAG47600.026.1341086
GAGTACT23850.022.70012312
ACGCAGA54750.022.6601587
CGCAGAG56250.021.8133098
AGAGTAC54100.021.54449811
TAACACG1004.358913E-420.6838784
CAGAGTA58900.020.14002810
GCAGAGT60450.019.7947569
GTACATG60950.019.6306881
GTATAAG6000.019.539231
AGTACTT28500.019.35940713
GTACTTT28000.018.84301414
GTATAGG4400.018.8078141
TACATGG62600.018.7768882
Produced by FastQC (version 0.11.5)