FastQCFastQC Report
Mon 6 Feb 2017
SRR2078214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755967
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18650.10620928525422176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20700.041.8714521
GTATCAA48700.040.293581
CGGTACG450.004630665830.65675225
ATCAACG67500.029.2622763
TCAACGC67950.029.1194844
TATCAAC67450.029.0801322
CAACGCA70150.028.156865
AACGCAG72450.027.4064796
ACGCAGA83800.023.326277
CGCAGAG86450.022.622158
CGTGCCG1854.8567017E-1022.3749710
GAGTACT57000.022.27029212
GTGCCGA1906.7666406E-1021.78615411
AGAGTAC84650.021.71966711
GTACATG61650.021.538431
AGTACTT61000.021.14923513
GCAGAGT91500.020.658569
CAGAGTA90500.020.54438210
GTACTTT60950.020.54403914
TACATGG63550.020.013012
Produced by FastQC (version 0.11.5)