FastQCFastQC Report
Mon 6 Feb 2017
SRR2078213_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078213_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520212
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA400.002595014234.50428465
GTATCAA18600.030.766761
GGTATCA9500.030.1190361
TCAACGC21300.026.54134
CAACGCA21650.026.270585
ATCAACG21700.026.2100493
TATCAAC22400.025.0807382
AACGCAG24150.023.9721556
GTTGCGT750.002004511422.9778548
ACGCAGA26350.021.8399077
ATTATAC3800.019.9564253
CGCAGAG28700.019.9354928
GTACATG34500.019.7848471
CGTGCCG2102.3665052E-919.70374310
AGAGTAC26300.019.40406411
GTACCGA2354.674803E-1019.0629636
TAATACT5450.018.975044
ATCGTCC1108.291908E-418.8000628
TACATGG35350.018.4277862
CGAATCG1903.4454752E-718.18473637
Produced by FastQC (version 0.11.5)