FastQCFastQC Report
Mon 6 Feb 2017
SRR2078213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520212
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10350.039.5224651
GTATCAA23850.039.389751
GCGATAC2200.026.65857114
TATCAAC34200.026.6529832
TGCGATA2350.026.42501613
TCAACGC33950.026.3344544
ATCAACG34000.026.2957293
CAACGCA34600.025.8397315
AACGCAG36750.024.516616
GTACGGG1052.19834E-523.1105941
GTGCGAT2900.021.41337412
ACGCAGA42300.021.1543337
CGATACT2950.021.04766515
GTACATG36750.020.9410271
GGTGCGA2850.020.57922211
TTTAGCG850.004016342620.3842853
CGCAGAG43900.020.1953168
CGTGCCG1901.5992555E-819.97395110
TAGTACT3850.019.8018784
TACATGG37950.019.5442122
Produced by FastQC (version 0.11.5)