FastQCFastQC Report
Mon 6 Feb 2017
SRR2078212_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078212_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477222
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA451.0050287E-438.3130410
GTATCAA34200.033.469441
GGTATCA17450.032.4029771
TCAACGC40650.026.7163874
CAACGCA41350.026.4308685
ATCAACG41800.026.2277473
TATCAAC43100.025.752972
AACGCAG44450.024.7321786
GCGATAC2450.023.92610414
ACGCAGA52000.021.0104067
GCGTAAC850.00413415820.28337511
GTACATG63950.020.217491
TGCGATA2900.020.21343213
TACATGG64000.019.6051462
AGAGTAC51350.019.4736211
CGCAGAG56200.019.3218778
TATACCG2002.906745E-818.9622735
GTGCGAT3400.018.25503712
ACATGGG68100.018.0730423
GAGTACT25100.017.85906612
Produced by FastQC (version 0.11.5)