FastQCFastQC Report
Mon 6 Feb 2017
SRR2078212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477222
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.041.6930431
GTATCAA50150.037.948581
ATCAACG70050.027.1031553
TATCAAC69850.027.0881542
TCAACGC70000.027.0730234
CAACGCA71200.026.5680775
AACGCAG74800.025.1967646
ACGCAGA86250.021.663197
CGCAGAG89300.020.8198438
GAGTACT61000.020.58854912
GTACATG60050.020.204551
AGAGTAC87700.019.86762411
TATACCG1601.3092267E-619.4881215
TACATGG62200.019.3326872
AGTACTT67300.018.8150413
CAGAGTA94500.018.73008510
GCAGAGT97550.018.4583199
GTACTTT68050.018.30346114
ACGTTCG1150.001114483917.99091544
ACATGGG64750.017.657193
Produced by FastQC (version 0.11.5)