FastQCFastQC Report
Mon 6 Feb 2017
SRR2078211_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078211_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2476790
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13000.036.611441
GTATCAA27800.033.0003051
ATCAACG34850.025.4322663
TATCAAC35800.025.3323062
TCAACGC34850.025.2348614
CAACGCA35500.024.96615
AACGCAG37650.023.7135376
CGAATCG2600.021.25841937
ACGCAGA44350.019.8230087
GTACATG56150.019.778221
CGCAGAG46350.018.7463938
TACATGG58000.018.4304432
TATTACG1150.001114445917.9910072
TGCGATA2501.0568328E-917.93357513
ACATGGG57900.017.6306323
CGTCGTA1200.001480977917.2438210
AGAGTAC45250.017.22476811
GTGTAGC12850.016.9090231
TCCGTTA3106.002665E-1116.6946665
GTATAGA5750.016.194851
Produced by FastQC (version 0.11.5)