FastQCFastQC Report
Mon 6 Feb 2017
SRR2078211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2476790
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42900.040.5581171
GGTATCA18500.036.533751
ATCAACG56800.030.0744533
TCAACGC57300.029.7515544
CAACGCA57800.029.4342385
TATCAAC58750.029.43132
AACGCAG61450.027.855076
GCGATAC2450.023.93434714
ACGCAGA72500.023.4459657
TAGTACG902.0658442E-423.0997854
GAGTACT51500.022.57155212
CGCAGAG75250.022.4173858
GGTGCGA2800.022.17491711
TGCGATA2650.022.12798113
TCGTTCG1103.2755866E-521.9487119
AGAGTAC73850.021.57938211
GTACTTT54550.020.86690114
AGTACTT55850.020.56647113
CAGAGTA79950.020.32122810
GCGCGTT1206.4149215E-520.11518336
Produced by FastQC (version 0.11.5)