FastQCFastQC Report
Mon 6 Feb 2017
SRR2078210_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078210_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093927
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.034.716321
GTATCAA14750.034.3648641
ATCAACG18750.026.4795593
TATCAAC18750.026.4771352
CAACGCA18900.026.2645955
TCAACGC19050.026.062564
CGTGCCG1359.493306E-925.5385310
AACGCAG19600.025.1380376
ACGCAGA23500.020.967157
GTATAGG2151.4006218E-1020.849431
CGCAGAG24350.020.2389438
ACTCGAC1206.347523E-520.14110246
TACGCTG1056.055418E-419.6984465
AGAGTAC23300.019.68001411
GCGATAC1601.3698136E-619.39420714
GTACATG26800.019.2994881
CGTTATG1651.8350474E-618.81081467
ATAAGAC2458.076313E-1018.2947653
GAGTACT12100.018.23577712
CAGAGTA25750.018.07532910
Produced by FastQC (version 0.11.5)