FastQCFastQC Report
Mon 6 Feb 2017
SRR2078210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093927
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12410.11344449858171524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20800.045.0073471
GGTATCA7850.038.4266551
CGTGCCG1500.034.4947410
TATCAAC30200.030.413212
ATCAACG30350.030.2573383
TCAACGC30400.030.207574
CAACGCA31100.029.6390825
AACGCAG33250.027.722576
TCGCTAG500.00775434127.58570154
GTGCCGA2200.025.08708611
ACGCAGA38250.023.66247
GCACCGT2600.022.6578716
CGCAGAG40100.022.6237348
AGAGTAC39900.021.35388811
CAGAGTA42150.020.45951310
GCAGAGT43300.020.1560489
GTACATG27500.019.9208281
GAGTACT27950.019.74654412
TACATGG27700.019.2692262
AGCACCG3450.019.084385
Produced by FastQC (version 0.11.5)