FastQCFastQC Report
Mon 6 Feb 2017
SRR2077544_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077544_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3996546
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62750.034.562661
GGTATCA32150.033.5146641
TCAACGC79700.026.7136944
CAACGCA80000.026.5275825
ATCAACG80300.026.4707833
TATCAAC80550.026.4301412
AACGCAG85100.024.8732286
ACGCAGA96450.021.6624367
GTACATG100450.020.4219761
CGCAGAG101550.020.3386388
CGAATCG3600.020.12857437
GAGTACT54600.020.08312212
TACATGG102150.019.9969252
TCCGTTA4350.019.83782465
AGAGTAC97600.019.71428911
CGTGCCG4450.019.37161810
TACCGAT4550.018.943737
GTACTTT58100.018.81606714
GTACCGA5450.018.3440346
CAGAGTA108700.018.14480810
Produced by FastQC (version 0.11.5)