FastQCFastQC Report
Mon 6 Feb 2017
SRR2077544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3996546
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36800.036.9430051
GTATCAA67100.034.1289521
TATCAAC92200.024.7854292
ATCAACG93000.024.3531483
TCAACGC93050.024.3403644
CAACGCA94550.023.9247955
CGAATCG3650.023.62203637
AACGCAG99800.022.697426
TCCGTTA4800.019.40129765
ACGCAGA118500.018.9391337
GTGGTAT18150.018.8688771
GTACATG93750.018.7078631
CGTGCCG5500.018.20700310
CGCAGAG122950.018.0856158
TACATGG94050.018.0488112
AGAGTAC119950.017.7042711
GAGTACT81150.017.61631212
TATACTG13750.017.330655
CAGAGTA129100.016.74369410
GTACTTT83850.016.66360514
Produced by FastQC (version 0.11.5)