FastQCFastQC Report
Mon 6 Feb 2017
SRR2077543_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077543_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4929471
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73350.035.804271
GGTATCA36850.034.7445531
TCAACGC92350.027.5635854
ATCAACG93800.027.3570143
TATCAAC94450.027.2771872
CAACGCA94150.027.039095
AACGCAG98200.025.9762276
ACGCAGA115250.021.7170127
GTACATG123600.021.4433731
CGCAGAG120700.020.5959078
TACATGG125800.020.2876632
AGAGTAC118550.019.77860311
GTACGCT7350.019.25325860
GTATAGC11850.018.638541
GAGTACT66100.018.51889812
ACATGGG133900.018.4429783
CAGAGTA130650.018.13065510
GCAGAGT136900.017.5546079
GTACTTT72300.017.1706914
TTGTACG8550.016.55121658
Produced by FastQC (version 0.11.5)