FastQCFastQC Report
Mon 6 Feb 2017
SRR2077543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4929471
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39900.037.7266351
GTATCAA84250.037.335821
TCAACGC120200.025.8697624
TATCAAC121000.025.8668162
ATCAACG121150.025.6676863
CAACGCA121500.025.5244965
AACGCAG127450.024.3818786
CGAATCG5250.021.67764337
GTACATG117050.020.7537921
ACGCAGA150400.020.524887
CGCACGA6400.020.47654335
CGCAGAG155350.019.7607568
AGAGTAC153000.019.04980311
GAGTACT105050.018.80353412
TACATGG123200.018.7519112
CGCGTAA950.00779439218.1754810
ACATGGG125050.017.9796053
CAGAGTA165550.017.85599510
GTACTTT111950.017.68848614
GCAGAGT171650.017.482279
Produced by FastQC (version 0.11.5)