FastQCFastQC Report
Mon 6 Feb 2017
SRR2077542_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077542_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268944
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41400.036.9407421
GGTATCA18300.036.4089781
TCAACGC51500.028.4749684
ATCAACG52300.028.2367043
CAACGCA53050.027.6429925
TATCAAC54900.027.0844042
AACGCAG55000.026.5767046
TATACCG1151.696737E-624.003455
ACGCAGA65350.022.3685257
CGCAGAG67150.021.821718
GTACATG59450.021.137411
AGAGTAC66350.020.83859811
GAGTACT42150.019.87848712
TACATGG61700.019.8498652
CAGAGTA71600.019.40651110
ACATGGG63200.018.7807663
GCAGAGT74650.018.65989
AGTACTT45550.018.3206113
CGAATCG1150.001109111418.0037837
GTACCGT1954.63373E-717.6792286
Produced by FastQC (version 0.11.5)