FastQCFastQC Report
Mon 6 Feb 2017
SRR2077542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268944
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.039.0726471
GTATCAA51750.034.3672871
TATCAAC68800.025.6310862
ATCAACG68850.025.515983
TCAACGC69250.025.4684724
CAACGCA71050.024.9339985
AACGCAG73800.023.9512216
GTGGTAT11150.021.4124661
CGATCGT1304.9424543E-621.22225242
GTACATG55100.020.5347141
ACGCAGA88200.019.9225127
TACATGG54800.019.8753722
TCGATCG1409.382407E-619.70637941
CGCAGAG89650.019.5617798
AGAGTAC85700.019.09422511
GAGTACT64550.019.0396212
ACATGGG58200.018.1823863
CAGAGTA94150.017.67346210
AGTACTT69950.017.6618813
TGGTATC12600.017.5628382
Produced by FastQC (version 0.11.5)