FastQCFastQC Report
Mon 6 Feb 2017
SRR2077541_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077541_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4529631
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38650.035.64441
GTATCAA75700.033.889161
TCAACGC98350.025.3988954
TATCAAC99050.025.3584522
ATCAACG99350.025.2121453
CAACGCA99150.025.0901245
AACGCAG104350.024.0491926
GTACATG123100.022.0180821
TACATGG124600.021.4046082
ACGCAGA118800.020.8062177
ACATGGG130850.019.7491823
CGCAGAG124900.019.626848
GAGTACT66950.018.85065812
AGAGTAC119450.018.84988411
TATACCG3200.018.3298635
GCGATAC4650.017.80026214
CAGAGTA132150.017.50763110
AGTACTT72250.017.37469713
GTGTAAG14800.017.2638591
CATGGGG103300.017.1343524
Produced by FastQC (version 0.11.5)