FastQCFastQC Report
Mon 6 Feb 2017
SRR2077541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4529631
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47900.038.2030031
GTATCAA90450.032.278341
TATCAAC127250.022.8504282
ATCAACG130250.022.2995553
TCAACGC130900.022.1890684
CAACGCA131700.022.0492955
AACGCAG137100.021.1549456
GTACATG114650.020.2755471
TACATGG116050.019.3653072
TAGATCG2002.85836E-818.990975
GCGATAC5450.018.35782814
ACGCAGA160150.017.8739977
ACATGGG125850.017.5841483
CGCAGAG166050.017.0519438
GTGGTAT25400.016.8874971
CGAATCG4750.016.69938937
AGAGTAC160950.016.62775611
CGTGCCG6750.016.3707710
CGTACAC4250.016.2718033
GCACCGT10250.016.169166
Produced by FastQC (version 0.11.5)