FastQCFastQC Report
Mon 6 Feb 2017
SRR2077540_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077540_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6241372
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87100.031.6328641
GGTATCA47450.030.8520051
TCAACGC110350.024.2314644
CAACGCA110450.024.1790645
ATCAACG111550.024.1248633
TATCAAC114450.023.6936842
AACGCAG117000.022.9539136
GTACATG156500.021.6646141
TACATGG158400.020.8440762
ACATGGG160850.019.7121623
ACGCAGA136550.019.2902667
CGCAGAG141450.018.5992938
AGAGTAC134350.018.12004711
CGTGCCG10000.016.89590310
GAGTACT74450.016.48823212
CATGGGG127750.016.4747644
CAGAGTA154550.016.0415110
ACTCGAC9400.015.78621846
GTACTTT77800.015.78003714
GCACCGT13900.015.6253926
Produced by FastQC (version 0.11.5)