FastQCFastQC Report
Mon 6 Feb 2017
SRR2077540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6241372
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104150.033.238171
GGTATCA57500.030.733741
TATCAAC144650.023.560262
TCAACGC143700.023.5262414
ATCAACG145100.023.2986893
CAACGCA145050.023.2314455
AACGCAG152650.022.1172166
GCGATAC7450.020.37442414
GTACATG148200.019.975051
TACATGG152350.019.238672
ACGCAGA176950.018.8657787
CGAATCG8050.018.85175137
ACATGGG156150.018.0416243
CGCAGAG184700.018.0002758
AGAGTAC173750.017.92348111
GCACCGT14600.017.7335956
GAGTACT114100.017.03583512
TGCGATA8200.016.83457613
AGTACTT121950.016.30034313
GTACTTT120650.016.212314
Produced by FastQC (version 0.11.5)