FastQCFastQC Report
Mon 6 Feb 2017
SRR2077539_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077539_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4929442
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36550.034.2882921
GTATCAA66700.033.0233731
TATCAAC86200.025.1729562
TCAACGC86450.025.0218734
ATCAACG87700.024.6256473
CAACGCA88100.024.474185
AACGCAG93700.022.8838026
GTACATG129500.021.2478561
TATATCG2001.2823875E-920.6992845
TACATGG130850.020.1687282
ACGCAGA108950.019.4930327
ACATGGG135750.018.857193
CGCAGAG114650.018.4060528
GCGATAC5650.017.7010514
AGAGTAC112800.017.4864311
GAGTACT55450.016.85369712
CGAATCG4600.016.5039637
CCGATAA3006.7848305E-1016.10402946
CAGAGTA125850.016.02873210
CATGGGG101300.015.8025014
Produced by FastQC (version 0.11.5)