FastQCFastQC Report
Mon 6 Feb 2017
SRR2077539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4929442
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41800.037.6512341
GTATCAA83900.034.671941
TATCAAC115850.024.7685642
TCAACGC116050.024.430464
CAACGCA116550.024.2340325
ATCAACG117000.024.2320943
AACGCAG123750.022.8221996
ACGCAGA146800.019.0954917
GTACATG125800.018.7245141
CGCAGAG153600.018.1609528
AGAGTAC151600.017.55883611
TACATGG128850.017.493712
GTGGTAT22050.017.4123951
GAGTACT96050.017.25378612
CGCGTTG3205.456968E-1217.24473237
ACATGGG134700.016.6329923
TGGTATC21050.016.5877722
CAGAGTA165900.016.2950610
AGTACTT101600.016.16993313
GTACTTT102550.016.14738514
Produced by FastQC (version 0.11.5)