FastQCFastQC Report
Mon 6 Feb 2017
SRR2077538_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077538_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5296406
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37600.033.8798331
GTATCAA73650.032.483531
CAACGCA97750.024.176015
TCAACGC97350.024.1681164
ATCAACG98350.023.9214743
TATCAAC99500.023.8521122
AACGCAG102550.023.0248056
CGAATCG7350.020.65853137
GTACATG134950.020.4909631
TACATGG137300.019.672192
ACGCAGA118950.019.649877
GCGATAC7500.018.85125514
ACTCGAC7900.018.78487846
CGCAGAG124200.018.5713798
ACATGGG144150.018.4748363
CGTTATG8800.018.43136667
AGAGTAC114700.018.36751211
CGTGCCG9250.017.89203310
TCCGTTA9100.017.82728465
GTATAGG12550.017.605121
Produced by FastQC (version 0.11.5)