FastQCFastQC Report
Mon 6 Feb 2017
SRR2077538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5296406
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47700.037.355931
GTATCAA91750.032.695181
TATCAAC124500.023.8002722
TCAACGC126200.023.4281984
ATCAACG126400.023.4184843
CAACGCA126450.023.3207455
AACGCAG132150.022.286836
CGTGCCG9550.020.9714310
GTACGCT8000.020.69328160
GCACCGT13050.019.8434526
GTACATG127750.019.7170121
TACATGG129500.018.876442
ACGCAGA156750.018.5907637
GTGCCGA10950.018.29015211
CGCAGAG162300.017.892068
TTGTACG8900.017.82550658
GAGTACT102750.017.7777712
AGAGTAC155350.017.69315311
CGAATCG8650.017.54194837
TGTACGC8850.017.53651659
Produced by FastQC (version 0.11.5)