FastQCFastQC Report
Mon 6 Feb 2017
SRR2077537_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077537_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4824351
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36100.034.333251
GTATCAA69000.033.0234531
TCAACGC87700.025.4141884
CAACGCA88300.025.1247985
ATCAACG88700.025.0493553
TATCAAC91050.024.7049432
AACGCAG93200.023.8195956
GTACATG113850.021.4091451
CGTACGC1004.336549E-420.7007223
CGAATCG4700.020.5598937
TACATGG117400.020.2766972
ACGCAGA108800.020.247557
CGCAGAG113800.019.2684568
ACATGGG122100.018.8753533
CGCGAAA1108.2688156E-418.80906914
AGAGTAC111550.018.39200211
TCCGTTA5150.018.09722365
CGTGCCG7200.017.71818710
CAGAGTA123650.016.98139210
GCGATAC5800.016.64710614
Produced by FastQC (version 0.11.5)