FastQCFastQC Report
Mon 6 Feb 2017
SRR2077537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4824351
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40400.038.1955951
GTATCAA81650.035.0860021
TCAACGC112350.025.3020294
TATCAAC113150.025.273852
CAACGCA112250.025.2562185
ATCAACG113200.025.1736623
AACGCAG119050.023.8099046
GTACATG115300.020.7052061
ACGCAGA139950.020.0800067
CGTGCCG6050.019.97277810
TACATGG118350.019.4008272
CGCAGAG144900.019.3706178
GAGTACT97100.018.66682412
AGAGTAC144600.018.4561711
GCACCGT10950.018.2876436
ACATGGG124100.018.1136993
CGAATCG5000.017.93369337
CGTACAC5400.017.9320473
GTGGTAT21050.017.5869881
GTACTTT102000.017.51933314
Produced by FastQC (version 0.11.5)