FastQCFastQC Report
Mon 6 Feb 2017
SRR2077536_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077536_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4416299
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59750.032.6432381
GGTATCA33300.029.959621
TCAACGC73850.025.6935274
CAACGCA74850.025.2114145
TATCAAC76500.025.1177462
ATCAACG75700.024.973333
AACGCAG80400.023.6266236
ACGCAGA93150.020.0612037
GTACATG103050.019.697541
CGAATCG2707.2759576E-1219.16959437
CGCAGAG97100.019.140558
TACATGG105050.018.8167882
AGAGTAC95150.018.33880211
CGTGCCG4600.017.99131610
ACATGGG106800.017.7334373
CAGAGTA105100.016.99560410
GTCTAGG11550.016.7374271
GAGTACT54200.016.67002112
GTGGTAT20300.016.3251741
GCAGAGT110700.016.260269
Produced by FastQC (version 0.11.5)