FastQCFastQC Report
Mon 6 Feb 2017
SRR2077536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4416299
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39100.035.216961
GTATCAA70450.031.7246691
TCAACGC96500.022.6123334
ATCAACG96950.022.4352723
CAACGCA97300.022.3231375
TATCAAC100500.021.9138182
AACGCAG101450.021.4053576
GTACATG94900.019.2492261
CGTGCCG4850.018.50866910
TACATGG97150.018.0430472
ACGCAGA120250.017.8277267
CGCAGAG125100.017.1653258
ACATGGG101500.016.8642753
CCGGTAT3900.016.80044758
AGAGTAC124300.016.30461511
GAGTACT85650.016.16443612
TCGCACG1958.997864E-615.92234222
GGACCGT4600.015.7610596
CAGAGTA132250.015.66387310
GACCGTG8000.015.5346677
Produced by FastQC (version 0.11.5)