##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077536_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4416299 Sequences flagged as poor quality 0 Sequence length 75 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.867249024579177 31.0 31.0 34.0 27.0 34.0 2 30.94552384247534 33.0 31.0 34.0 27.0 34.0 3 31.03127845284026 33.0 31.0 34.0 27.0 34.0 4 34.435593921516634 37.0 35.0 37.0 31.0 37.0 5 34.16004124720722 37.0 35.0 37.0 30.0 37.0 6 34.14435390357401 37.0 35.0 37.0 30.0 37.0 7 33.982116699978874 37.0 35.0 37.0 30.0 37.0 8 33.92774651353996 37.0 35.0 37.0 29.0 37.0 9 35.40977162098852 39.0 35.0 39.0 29.0 39.0 10 35.2899070013149 39.0 35.0 39.0 28.0 39.0 11 35.31017872657626 39.0 35.0 39.0 29.0 39.0 12 35.202043838064405 39.0 35.0 39.0 28.0 39.0 13 35.15704688473312 39.0 35.0 39.0 27.0 39.0 14 36.39419590928966 40.0 37.0 41.0 27.0 41.0 15 36.438282598166474 40.0 37.0 41.0 28.0 41.0 16 36.41107021965678 40.0 37.0 41.0 28.0 41.0 17 36.39150813837559 40.0 37.0 41.0 28.0 41.0 18 36.387390210671875 40.0 36.0 41.0 28.0 41.0 19 36.41597976042836 40.0 37.0 41.0 27.0 41.0 20 36.375193119849904 40.0 37.0 41.0 27.0 41.0 21 36.34403648847145 40.0 37.0 41.0 27.0 41.0 22 36.326890230937714 40.0 37.0 41.0 27.0 41.0 23 36.300742544832225 40.0 37.0 41.0 27.0 41.0 24 36.28350367581543 40.0 36.0 41.0 27.0 41.0 25 36.25528434555722 40.0 36.0 41.0 27.0 41.0 26 36.18613889141111 40.0 36.0 41.0 27.0 41.0 27 36.13002380500052 40.0 36.0 41.0 27.0 41.0 28 36.10883751303977 40.0 36.0 41.0 27.0 41.0 29 36.05800648914396 40.0 36.0 41.0 27.0 41.0 30 35.98839933618625 40.0 36.0 41.0 27.0 41.0 31 35.910642599153725 40.0 36.0 41.0 27.0 41.0 32 35.85249141871961 40.0 36.0 41.0 27.0 41.0 33 35.80134995388673 40.0 36.0 41.0 27.0 41.0 34 35.74441698807078 40.0 35.0 41.0 26.0 41.0 35 35.669988830013544 40.0 35.0 41.0 26.0 41.0 36 35.6187866808837 40.0 35.0 41.0 26.0 41.0 37 35.56675918002834 40.0 35.0 41.0 25.0 41.0 38 35.51324468746342 40.0 35.0 41.0 25.0 41.0 39 35.44209234927255 40.0 35.0 41.0 25.0 41.0 40 35.38514421238236 40.0 35.0 41.0 25.0 41.0 41 35.34212289521158 40.0 35.0 41.0 25.0 41.0 42 35.28894352488362 40.0 35.0 41.0 25.0 41.0 43 35.1977345736781 40.0 35.0 41.0 24.0 41.0 44 35.087767155258284 40.0 35.0 41.0 24.0 41.0 45 35.008815299869866 39.0 35.0 41.0 24.0 41.0 46 34.89879195226591 39.0 35.0 41.0 23.0 41.0 47 34.774104742455165 39.0 35.0 41.0 23.0 41.0 48 34.68789522629695 39.0 35.0 41.0 22.0 41.0 49 34.60854688507277 39.0 34.0 41.0 22.0 41.0 50 34.613355209871436 39.0 34.0 41.0 22.0 41.0 51 34.53956853917726 39.0 34.0 41.0 22.0 41.0 52 34.4763712783034 39.0 34.0 41.0 21.0 41.0 53 34.38132359244698 39.0 34.0 41.0 20.0 41.0 54 34.35449932171712 39.0 34.0 41.0 20.0 41.0 55 34.27287916873382 39.0 34.0 41.0 20.0 41.0 56 34.14853636495174 39.0 34.0 41.0 19.0 41.0 57 34.06968029112159 39.0 34.0 41.0 19.0 41.0 58 33.969725555266976 38.0 34.0 41.0 18.0 41.0 59 33.87585170297573 38.0 34.0 41.0 18.0 41.0 60 33.84656269876655 38.0 34.0 41.0 18.0 41.0 61 33.734502124969346 38.0 34.0 40.0 18.0 41.0 62 33.58790901612414 38.0 34.0 40.0 15.0 41.0 63 33.45651913514008 37.0 34.0 40.0 13.0 41.0 64 33.30224448118209 37.0 33.0 40.0 13.0 41.0 65 33.17314792318183 37.0 33.0 40.0 11.0 41.0 66 32.97382808546251 37.0 33.0 40.0 8.0 41.0 67 32.78260190263386 36.0 33.0 39.0 6.0 41.0 68 32.56762234622248 36.0 33.0 39.0 2.0 41.0 69 32.4009601704957 36.0 33.0 39.0 2.0 41.0 70 32.20803369518232 36.0 33.0 39.0 2.0 41.0 71 32.02628807515071 35.0 33.0 39.0 2.0 41.0 72 31.822241202418585 35.0 32.0 39.0 2.0 41.0 73 31.622109599010393 35.0 32.0 38.0 2.0 40.0 74 31.35642401024025 35.0 32.0 37.0 2.0 40.0 75 31.12849809308654 35.0 32.0 37.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48492372170061415 1101 2 0.5099149346210261 1101 3 0.519405164431614 1101 4 0.5608528975330955 1101 5 0.5819324505013626 1101 6 0.6138299790930475 1101 7 0.6599064625334492 1101 8 0.6658363065122472 1101 9 0.8125227976202751 1101 10 0.7247990670849376 1101 11 0.757500279242258 1101 12 0.8799767578063395 1101 13 0.7933599013205992 1101 14 0.960603951467327 1101 15 0.8360571224472437 1101 16 0.7989613529737198 1101 17 0.9561692067295411 1101 18 0.8322200626317553 1101 19 0.9607283488523493 1101 20 0.963099888705635 1101 21 0.9328687452949112 1101 22 0.9660769337770816 1101 23 0.9252774870387697 1101 24 0.9014252055066976 1101 25 0.993258117971834 1101 26 1.001985713932065 1101 27 0.9652324968021375 1101 28 1.0747054344872495 1101 29 1.0182699727443563 1101 30 0.9985555602205238 1101 31 1.0709414427719182 1101 32 1.0228819319295042 1101 33 1.0155656350489366 1101 34 0.9583030919593227 1101 35 1.1224852225757473 1101 36 1.052405240824669 1101 37 1.0522346956361943 1101 38 1.0924062046852185 1101 39 1.130590593636441 1101 40 1.081378127109737 1101 41 1.062041421504972 1101 42 1.0305728554477867 1101 43 1.0858443266819222 1101 44 1.1053249616625536 1101 45 1.0752791983216525 1101 46 0.980318637732779 1101 47 1.065962542668295 1101 48 1.045325077314061 1101 49 1.0568927438363573 1101 50 0.9719598737891104 1101 51 0.9851889942673608 1101 52 0.9874865923730667 1101 53 0.9763975970476224 1101 54 1.012789112958714 1101 55 0.9662512421915466 1101 56 1.0295461202312879 1101 57 0.8919041599401893 1101 58 0.9698645784304603 1101 59 0.9157704384398713 1101 60 0.9258201558365187 1101 61 0.8412240388586909 1101 62 0.9169836716347035 1101 63 0.9486962936918104 1101 64 0.9300797623038122 1101 65 0.855653976712837 1101 66 0.9550136238527926 1101 67 1.008749698574114 1101 68 0.9822799583456998 1101 69 0.9541116115229542 1101 70 0.9288901843000801 1101 71 0.9383875856032304 1101 72 0.9797669504455371 1101 73 0.9934377150655429 1101 74 1.0533091343286394 1101 75 1.0476932712342055 1102 1 0.605406243466625 1102 2 0.6105147681165022 1102 3 0.6261866522133808 1102 4 0.6034325418809559 1102 5 0.656384752391638 1102 6 0.7135269964875235 1102 7 0.8367360368722814 1102 8 0.8258539810243519 1102 9 0.9712889700200904 1102 10 0.9301200055029142 1102 11 1.0062598078966474 1102 12 1.0534227629936481 1102 13 1.046938326278834 1102 14 1.1638441522588892 1102 15 1.0727565147752713 1102 16 1.128023093028169 1102 17 1.121847655772143 1102 18 1.146832952256453 1102 19 1.1173104926644655 1102 20 1.1113278545121545 1102 21 1.1863321215692224 1102 22 1.1863367541620633 1102 23 1.1885073587342063 1102 24 1.2269124714505821 1102 25 1.2387654352577755 1102 26 1.1577756653777271 1102 27 1.2164291611488665 1102 28 1.178556467934179 1102 29 1.1967739479292447 1102 30 1.1683583149411518 1102 31 1.2422356582884504 1102 32 1.2458215016600676 1102 33 1.241682444862711 1102 34 1.2335647803050804 1102 35 1.3382633277997797 1102 36 1.287539591769935 1102 37 1.2744164611783546 1102 38 1.224712191199238 1102 39 1.2999995278620986 1102 40 1.3159501302021397 1102 41 1.3218800119942387 1102 42 1.2567306276501284 1102 43 1.2752559285199183 1102 44 1.2378816531658288 1102 45 1.2877511077947688 1102 46 1.2820894392617888 1102 47 1.330553149512177 1102 48 1.274179160815386 1102 49 1.194605639213485 1102 50 1.1880941242248113 1102 51 1.2514851030300918 1102 52 1.2227180393504824 1102 53 1.2340331948804106 1102 54 1.260724083902602 1102 55 1.2182868769410575 1102 56 1.2552779594488541 1102 57 1.2566178435904476 1102 58 1.2106565432280902 1102 59 1.2909575979953303 1102 60 1.334006565046991 1102 61 1.302417548848645 1102 62 1.2643189355102393 1102 63 1.2623152132582405 1102 64 1.2748984942523904 1102 65 1.1981996753775732 1102 66 1.2270878315181974 1102 67 1.2490144622596944 1102 68 1.2790090989762817 1102 69 1.2956616965768006 1102 70 1.2602068785594653 1102 71 1.301514988113695 1102 72 1.3204633995500856 1102 73 1.3155584795197974 1102 74 1.3731148916934934 1102 75 1.3549379880026784 1103 1 0.5255202097897396 1103 2 0.5785985368312367 1103 3 0.6101780138123338 1103 4 0.4994952929394927 1103 5 0.5220441245107423 1103 6 0.5658644937028114 1103 7 0.6679153010302414 1103 8 0.6151078705042821 1103 9 0.7345087792813487 1103 10 0.7921856103189455 1103 11 0.8402152528147511 1103 12 0.8250996621699613 1103 13 0.7920001099279759 1103 14 0.9397577927786145 1103 15 0.9371141121232753 1103 16 0.9566550383890586 1103 17 1.0201167323885372 1103 18 1.0033462898990777 1103 19 1.0168824762146116 1103 20 1.0334329411420597 1103 21 0.9759346227995636 1103 22 0.981857376209966 1103 23 1.010175725413852 1103 24 1.0767209295783147 1103 25 1.0358574047219307 1103 26 1.0568274673620053 1103 27 1.069739327603898 1103 28 1.0547658033936145 1103 29 1.0243887394927924 1103 30 1.0047340405719112 1103 31 1.0261301020469134 1103 32 1.0528579397254063 1103 33 1.0297701832227588 1103 34 1.0716448117037274 1103 35 1.1096180087675478 1103 36 1.1381282043128564 1103 37 1.189357670684231 1103 38 1.2333303076650424 1103 39 1.2003177481212788 1103 40 1.156668218517197 1103 41 1.135635528781087 1103 42 1.1258999162757775 1103 43 1.1693725391504373 1103 44 1.2593645574507946 1103 45 1.2220737154201018 1103 46 1.211337976914514 1103 47 1.3004396749722176 1103 48 1.2764329192710804 1103 49 1.2764334497748422 1103 50 1.2282156099948836 1103 51 1.278336250883811 1103 52 1.2699125697783558 1103 53 1.2508240869149034 1103 54 1.3550576923750413 1103 55 1.2972622980436483 1103 56 1.351763077937342 1103 57 1.3202094385564251 1103 58 1.2663391565236708 1103 59 1.3341953614693338 1103 60 1.2589906941314553 1103 61 1.2692458435274716 1103 62 1.2757448389436092 1103 63 1.2462773037352548 1103 64 1.3386652678022841 1103 65 1.2964669358163832 1103 66 1.2650188184208417 1103 67 1.217621549574318 1103 68 1.208241975045631 1103 69 1.3119251659217852 1103 70 1.3832851533369919 1103 71 1.2978339499394096 1103 72 1.3239655088516642 1103 73 1.3002906057082875 1103 74 1.3278752995409384 1103 75 1.330427020353877 1104 1 0.4875627220245917 1104 2 0.5719667753112496 1104 3 0.5500812329582132 1104 4 0.6039166340548903 1104 5 0.6338381535861046 1104 6 0.6740905958672343 1104 7 0.6625937332761396 1104 8 0.7281734210283801 1104 9 0.7988333503600558 1104 10 0.8834695866877311 1104 11 0.8471660609512455 1104 12 0.8312625556489621 1104 13 0.8448209512239515 1104 14 0.964252716766552 1104 15 0.9398439585387379 1104 16 0.9337645423003025 1104 17 0.9152363265293317 1104 18 0.9538397743573412 1104 19 0.9368114435515125 1104 20 1.022986953320597 1104 21 0.9491345717953763 1104 22 0.9885105021569132 1104 23 1.0554521520268452 1104 24 1.0626986313365947 1104 25 1.0945224985340758 1104 26 1.0640134194129303 1104 27 1.0919266906535299 1104 28 1.0362521752555338 1104 29 1.0630275226074843 1104 30 1.0662072043501212 1104 31 1.019624769256076 1104 32 1.0928279429882153 1104 33 1.075143290628624 1104 34 1.0759785451468034 1104 35 1.096639457435252 1104 36 1.104047413515353 1104 37 1.1285334851793394 1104 38 1.0977442172050829 1104 39 1.1480533960603978 1104 40 1.1754769597048806 1104 41 1.1397754949847254 1104 42 1.1036281772614487 1104 43 1.1293727203424098 1104 44 1.149378459202282 1104 45 1.0664183149729851 1104 46 1.2190389063076523 1104 47 1.2066545056435842 1104 48 1.1580973866187279 1104 49 1.1933843106463158 1104 50 1.1769769532548295 1104 51 1.1644192332929109 1104 52 1.1557435736454522 1104 53 1.173414615631735 1104 54 1.1804185787878225 1104 55 1.1518651353747629 1104 56 1.2198829042472568 1104 57 1.1839145359058705 1104 58 1.1591446316583855 1104 59 1.1635209716975368 1104 60 1.0976254477529608 1104 61 1.1588275212867387 1104 62 1.1228278712481057 1104 63 1.1428423095340747 1104 64 1.1672329817450517 1104 65 1.105218129621342 1104 66 1.0969727940364606 1104 67 1.1608556586662147 1104 68 1.156763171329139 1104 69 1.15441667187185 1104 70 1.1227243993295772 1104 71 1.1394562752787962 1104 72 1.1725055139439675 1104 73 1.091427099922111 1104 74 1.1034164381648317 1104 75 1.150689516143526 1105 1 0.4275774467188569 1105 2 0.46333666652735417 1105 3 0.48117016326797213 1105 4 0.49758482712486085 1105 5 0.4799737515991467 1105 6 0.5536003785136927 1105 7 0.5575510161967614 1105 8 0.6080875106216297 1105 9 0.7043030520277043 1105 10 0.770343907119738 1105 11 0.6593719465966075 1105 12 0.7666271370723194 1105 13 0.7611712547148528 1105 14 0.7686852887471431 1105 15 0.7742083008742071 1105 16 0.7859219610589463 1105 17 0.7925948439618651 1105 18 0.7777410095483575 1105 19 0.8747279387448046 1105 20 0.8400144008464565 1105 21 0.8877735444000763 1105 22 0.9041248338129506 1105 23 0.8484255049656468 1105 24 0.8121194335949653 1105 25 0.855756288412671 1105 26 0.926425701826858 1105 27 1.0508759535053116 1105 28 0.9695332483671351 1105 29 0.9750396756501942 1105 30 0.920063124128049 1105 31 0.9520665593299 1105 32 0.9329497380462115 1105 33 0.8766463459544909 1105 34 0.9547976148526374 1105 35 0.9418535352909743 1105 36 0.961862084521087 1105 37 0.9592314189508997 1105 38 0.963477245021167 1105 39 0.9875111822397713 1105 40 0.9815494108262612 1105 41 0.9368054337213536 1105 42 1.0266820108540884 1105 43 0.9864378374637113 1105 44 1.039661567386858 1105 45 1.0464059097422833 1105 46 1.0134500068203849 1105 47 1.0268868568326184 1105 48 1.0692443192902772 1105 49 1.0755881366395812 1105 50 1.0072614012428787 1105 51 1.059257278962157 1105 52 1.013802327226017 1105 53 1.0050902379075808 1105 54 1.0379450474641345 1105 55 0.9983775257309304 1105 56 1.053470944823239 1105 57 1.033324174595748 1105 58 1.0188184880925277 1105 59 1.0521195771365015 1105 60 1.0995804304439574 1105 61 1.1152788918754553 1105 62 1.1661277958063963 1105 63 1.1103569166997929 1105 64 1.1679898743175627 1105 65 1.1467762220408844 1105 66 1.1190105238310437 1105 67 1.0412104857682607 1105 68 1.1122132162660066 1105 69 1.0602953246709816 1105 70 1.0670454661881763 1105 71 1.064660914048197 1105 72 1.0690800465582626 1105 73 1.0400649145475462 1105 74 1.118358226157543 1105 75 1.079593291835014 1106 1 0.33385255567919003 1106 2 0.4121706761070705 1106 3 0.39155456417365997 1106 4 0.4395337308052589 1106 5 0.34440300323232265 1106 6 0.4519032810277821 1106 7 0.4325342174910318 1106 8 0.5123667329040913 1106 9 0.6258803590122639 1106 10 0.6681677530992971 1106 11 0.6217052020915617 1106 12 0.6665668535944107 1106 13 0.5639187673112787 1106 14 0.6466957620501503 1106 15 0.5773105343941083 1106 16 0.6818775572965379 1106 17 0.686200261341618 1106 18 0.6370253179076215 1106 19 0.6375744173427123 1106 20 0.6975456842757168 1106 21 0.7254689032453996 1106 22 0.7287464317789372 1106 23 0.7074161025995522 1106 24 0.7097846659217311 1106 25 0.7553889388498121 1106 26 0.8229244731873635 1106 27 0.7893018247962189 1106 28 0.736281442751455 1106 29 0.66429690092653 1106 30 0.6773406535811759 1106 31 0.745292858138086 1106 32 0.747342802657279 1106 33 0.7565336438691972 1106 34 0.7772026089939672 1106 35 0.7577551338589217 1106 36 0.7590924616230694 1106 37 0.7323548146061754 1106 38 0.6170965185329038 1106 39 0.7457241227943356 1106 40 0.8185070950660744 1106 41 0.6561878913257502 1106 42 0.6829793662420727 1106 43 0.7995225055773432 1106 44 0.8154246080648164 1106 45 0.7888760969031807 1106 46 0.7252411536918473 1106 47 0.7902492210305354 1106 48 0.7289017373811362 1106 49 0.8050414357446058 1106 50 0.7187797815082959 1106 51 0.8051379919342736 1106 52 0.7077389017872235 1106 53 0.7220170243192996 1106 54 0.7521623679764105 1106 55 0.7826038342072792 1106 56 0.7928847268554904 1106 57 0.7925601828575495 1106 58 0.7589947904153362 1106 59 0.7539331770899693 1106 60 0.6780294590280462 1106 61 0.7383557870840534 1106 62 0.7633934542098686 1106 63 0.7481122953457842 1106 64 0.7312623695932956 1106 65 0.7174395690324857 1106 66 0.6874606755087029 1106 67 0.6897243247183553 1106 68 0.6881269988663448 1106 69 0.6887173434872906 1106 70 0.7171473067003618 1106 71 0.7331881988227735 1106 72 0.7268563740397411 1106 73 0.6637736275948747 1106 74 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-1.9874193855417843 2216 12 -1.6178940043969448 2216 13 -1.6988380486388834 2216 14 -1.8904034225655693 2216 15 -1.7279891440111115 2216 16 -1.853909667601087 2216 17 -2.0890290961544338 2216 18 -1.8949553796874454 2216 19 -2.091119887019957 2216 20 -2.1315503550614707 2216 21 -2.151297772029352 2216 22 -2.154343467823871 2216 23 -2.274118986428306 2216 24 -2.2621702851224796 2216 25 -2.278437887604589 2216 26 -2.2620998030070254 2216 27 -2.2242392855471707 2216 28 -2.1283426896806006 2216 29 -2.1210324388330335 2216 30 -2.2018921371395805 2216 31 -2.1733266493725196 2216 32 -2.162240779879248 2216 33 -2.008633404102227 2216 34 -2.126509097605478 2216 35 -2.0715038466601214 2216 36 -2.1421557639876383 2216 37 -2.1052165371990483 2216 38 -2.1175325594340535 2216 39 -2.1315697073791995 2216 40 -2.0435434360635654 2216 41 -1.971661364766085 2216 42 -2.030407292457994 2216 43 -2.0973870323632227 2216 44 -1.9692390043438195 2216 45 -2.1019454078816864 2216 46 -2.040541435219609 2216 47 -2.2320490228468373 2216 48 -2.235843231019537 2216 49 -2.207292236972684 2216 50 -2.1033324433415785 2216 51 -2.074463820387912 2216 52 -2.1035098750836667 2216 53 -2.127555926864936 2216 54 -2.2832926189293516 2216 55 -2.233527474519299 2216 56 -2.425416939972205 2216 57 -2.2904928606881185 2216 58 -2.312629450622225 2216 59 -2.361989858031361 2216 60 -2.2879249887031 2216 61 -2.217546427921512 2216 62 -2.2052950991325915 2216 63 -2.2118795852569235 2216 64 -2.219379031837864 2216 65 -2.2240953039698823 2216 66 -2.257532383684328 2216 67 -2.228793624464675 2216 68 -2.2749688651477094 2216 69 -2.326768786655034 2216 70 -2.2494222209407937 2216 71 -2.224312143821326 2216 72 -2.260498692117082 2216 73 -2.179678820747867 2216 74 -2.2083937941383667 2216 75 -2.1971592169567487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96268.0 3 35943.0 4 21177.0 5 13561.0 6 10164.0 7 9271.0 8 8748.0 9 8896.0 10 9531.0 11 10254.0 12 11042.0 13 11806.0 14 12541.0 15 13009.0 16 13773.0 17 14419.0 18 15259.0 19 16572.0 20 18616.0 21 21315.0 22 25918.0 23 30845.0 24 33642.0 25 36457.0 26 39121.0 27 43261.0 28 49939.0 29 59506.0 30 69124.0 31 80047.0 32 96838.0 33 120379.0 34 155195.0 35 208849.0 36 292890.0 37 456709.0 38 763450.0 39 1185777.0 40 296187.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.174723127035826 18.368888259453335 14.019430675541708 26.436957937969126 2 15.565533408355956 19.426835617794087 37.94481647502229 27.06281449882767 3 18.396569171682486 23.145478469804196 29.0474850170724 29.41046734144092 4 12.62994043475599 15.947681444984344 35.810751673926205 35.61162644633346 5 14.06709689234778 36.98905789460801 34.32257134490253 14.62127386814169 6 33.8148957600436 36.02495731492465 16.412686219660173 13.747460705371578 7 29.19925471612213 31.290529793914423 20.577595005347725 18.932620484615722 8 26.613563900326316 35.291943284469816 18.955413562631946 19.139079252571918 9 25.962938697245097 15.726178792829874 18.79346873603903 39.517413773886 10 14.239932195005498 29.266906061906035 33.504582318425264 22.988579424663204 11 35.92326808182207 22.615732788052128 22.488478866088062 18.972520264037733 12 23.365227861667268 24.545564173193362 29.957902124025075 22.131305841114294 13 28.420063076726425 21.408420443821555 25.51590924131154 24.65560723814048 14 21.864700094853426 20.83041942793892 26.87747823547709 30.427402241730565 15 23.835223547439327 28.967983184541772 23.309480029378584 23.887313238640314 16 23.701405113766988 27.94449307975524 25.047378846169092 23.30672296030868 17 22.47371994729268 27.803564221532 26.458521975500787 23.26419385567453 18 22.47718317170163 27.293159469694313 27.317754695910374 22.911902662693684 19 23.351058734328916 26.855941428952214 27.517784848009274 22.275214988709596 20 23.4262521805632 26.25505665331361 27.582111806531078 22.736579359592113 21 23.568273307496074 26.059467673339793 27.61774385961177 22.754515159552362 22 23.3025075799919 25.99611103612397 27.488329937774765 23.213051446109358 23 23.154721342965413 26.029262663053167 27.290772547983188 23.525243445998232 24 22.887505129833478 26.14699905873784 27.514954653630923 23.450541157797755 25 23.19969417498925 26.017410556262266 27.567053786837885 23.2158414819106 26 22.994656645895763 26.295032877823154 27.604573675543563 23.105736800737514 27 23.107756787205794 26.46846046136374 27.307260388339927 23.11652236309054 28 23.013488617683414 26.39290302135341 27.407873536309314 23.18573482465386 29 23.172151778290782 26.380188805342915 27.369819690217874 23.07783972614843 30 23.236129077852574 26.346422131230163 27.355529466500467 23.061919324416795 31 23.184723704402938 26.05154304826544 27.403941100906458 23.359792146425164 32 23.035182912192184 26.33369416048807 27.34345913972163 23.287663787598113 33 23.079712097790008 26.15852285929235 27.381033801016653 23.380731241900996 34 23.25235325356484 26.33907006711288 27.357278978733813 23.05129770058847 35 23.332783818167243 26.22362610263464 27.33317874445144 23.110411334746676 36 23.27629780399851 26.339041868326866 27.234884203336897 23.149776124337727 37 23.251901582612213 26.02726449055883 27.424004065284596 23.29682986154436 38 23.16230649600968 26.21054629014888 27.437554741781828 23.189592472059616 39 23.328624606366883 26.492250528429672 27.157662037802368 23.021462827401077 40 23.374306721352202 26.13172764212453 27.426806464886376 23.067159171636895 41 23.155480199837086 26.232161487495915 27.252543538572993 23.35981477409401 42 23.360178986115216 26.248051114304054 27.203149467219657 23.18862043236107 43 23.185707317227827 26.058302485756823 27.33177559299588 23.42421460401948 44 23.214337071455958 26.400403274293776 27.069515221634415 23.31574443261585 45 23.34695781543318 26.292019827584255 27.021446670908748 23.339575686073815 46 23.355346135539502 26.24229600984372 26.839118902191878 23.563238952424904 47 23.186949975464515 26.348234498091394 26.940754560690284 23.524060965753808 48 23.10671129784427 26.424135980467593 27.0398904359239 23.42926228576423 49 23.336955583991116 26.32801830885804 26.852606854318722 23.482419252832116 50 23.478722402162177 26.312799346419435 26.829205885123436 23.379272366294952 51 23.186420029844594 26.635938961235993 26.6009994995709 23.576641509348516 52 23.28529640735241 26.586470769120684 26.629245235594695 23.49898758793221 53 23.30107177978665 26.48783517601503 26.7826521709694 23.42844087322892 54 23.254947185414757 26.56708705637911 26.81799850961178 23.359967248594355 55 23.220632350064125 26.3669171938062 26.807785180676625 23.604665275453048 56 23.12957012257949 26.677649680360332 26.7144937283595 23.478286468700677 57 23.299498569800786 26.46276138045473 26.619571538039317 23.61816851170517 58 23.10186916988279 26.37316554249583 26.849017229838967 23.67594805778241 59 23.228609905703852 26.529597840208098 26.781319937744296 23.460472316343754 60 23.222386086997478 26.611330037771925 26.81159075712093 23.35469311810967 61 23.26123998446651 26.36714508753438 26.88901141575517 23.482603512243944 62 23.093864290910542 26.614760417714166 26.785463532451487 23.505911758923805 63 22.952197330376748 26.419913114053205 26.927442263237605 23.70044729233244 64 23.145000558224897 26.417172220645806 26.818233595264985 23.619593625864315 65 23.17194969385287 26.339025673963047 26.828684432470528 23.660340199713556 66 23.083752140462828 26.504755841728162 26.70206003056947 23.709431987239547 67 23.132544636528056 26.42950352322721 26.72052023370114 23.717431606543595 68 23.16647567454943 26.422204305702135 26.769908097310864 23.641411922437573 69 23.20675468567143 26.476217477472908 26.613875493204738 23.70315234365093 70 23.09349353955778 26.225382401924797 26.866240514969284 23.81488354354814 71 23.118292109896455 26.307032988184943 26.797117284404916 23.777557617513686 72 22.98864293129594 26.487189893232504 26.649433731861937 23.87473344360962 73 23.18115313710167 26.258279322939394 26.747118583885626 23.81344895607331 74 23.15819177946926 26.359944168859535 26.501424427867832 23.980439623803377 75 23.113342768185678 26.422258703651284 26.669255590150016 23.79514293801302 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 487.0 1 339.5 2 260.5 3 346.5 4 364.0 5 289.0 6 298.0 7 663.5 8 945.0 9 1574.5 10 2526.0 11 2957.5 12 3067.0 13 2929.0 14 2929.5 15 3476.5 16 3885.0 17 4306.0 18 5248.5 19 6613.0 20 7456.0 21 8730.0 22 11607.0 23 15577.0 24 17944.0 25 21010.0 26 28584.5 27 37737.0 28 42381.0 29 48487.5 30 60429.5 31 73501.0 32 80737.0 33 87764.0 34 102712.5 35 118513.0 36 126392.0 37 132854.5 38 144682.0 39 156013.0 40 161979.0 41 168873.5 42 181281.0 43 192024.5 44 197255.0 45 203673.5 46 213477.0 47 219917.0 48 222972.0 49 224737.0 50 225058.5 51 222043.0 52 220471.0 53 216320.5 54 207049.5 55 194003.0 56 186077.0 57 176404.0 58 155452.5 59 131637.5 60 119101.0 61 107312.0 62 84375.5 63 63637.0 64 54046.0 65 45175.0 66 30621.5 67 21841.5 68 18744.0 69 15630.5 70 10472.0 71 7040.5 72 5654.0 73 5052.5 74 3882.0 75 2501.5 76 1690.0 77 1474.5 78 956.0 79 511.5 80 370.0 81 300.0 82 172.5 83 89.5 84 64.0 85 48.0 86 22.0 87 8.0 88 4.0 89 3.5 90 5.5 91 4.5 92 1.0 93 2.5 94 2.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07187013379302443 2 0.016801398637184665 3 0.0024001998053120948 4 0.1360641568879281 5 0.09852140898974457 6 0.07164369984912707 7 0.03027421829907803 8 0.027443794000360934 9 0.049973971418149 10 0.02909676179081172 11 0.016257957171830983 12 0.10585786877201928 13 0.08819602114802463 14 0.048003996106241904 15 0.018975164498599394 16 0.03065915600370355 17 0.022032022741213853 18 0.01829586266690729 19 0.013631323422621522 20 0.013178455534826786 21 0.02780608831059672 22 0.016461747721338613 23 0.014446485620652044 24 0.021533868064639645 25 0.015895662861595196 26 0.03326314635852328 27 0.029459056101047508 28 0.038222049729875626 29 0.05047212609472321 30 0.03410095195094354 31 0.019994117246137547 32 0.01175192168827337 33 0.014333268648703358 34 0.013676610211400996 35 0.006453367401074972 36 0.010302744447330219 37 0.00842334271298207 38 0.002513416777260779 39 0.008717706840048647 40 0.011457557561206793 41 0.011366983983647846 42 0.007110025838377338 43 0.011344340589258109 44 0.010098953897822588 45 0.005185337315249715 46 0.0018341149455686765 47 0.005570275019875239 48 0.0057287787806033965 49 0.0017208979736199927 50 2.4907733828710417E-4 51 0.0020152621006865705 52 0.0025587035660402523 53 0.0 54 0.0 55 2.4907733828710417E-4 56 0.008808280417607594 57 0.0029662846650555137 58 1.5850376072815722E-4 59 0.007200599415936285 60 0.0012680300858252578 61 7.698754092510493E-4 62 0.023345339615818583 63 0.008944140783946015 64 0.011683991505104161 65 0.01754863065204598 66 0.017707134412774137 67 0.016869328820353875 68 0.017186336341810188 69 0.006408080612295499 70 0.014876710114057043 71 0.007336459782274706 72 0.016371174143779667 73 0.007427033359833653 74 0.01906573807615834 75 0.0011095263250971007 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 4416299.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.702021730847854 #Duplication Level Percentage of deduplicated Percentage of total 1 75.08460066145454 44.827024603421926 2 13.546922888726876 16.175573693777842 3 5.013257637339856 8.979048492204091 4 2.289282358916834 5.466991405603979 5 1.2380705146092523 3.6957656383761783 6 0.7469335138714003 2.675606452598933 7 0.4863237382099112 2.0324157270784697 8 0.3200370055054435 1.528548500588917 9 0.2317437093848457 1.2452011176313211 >10 0.9809184827507991 9.808531043139757 >50 0.0452361451976251 1.8613869771517042 >100 0.01617833924910702 1.4831243465315254 >500 3.807729951798663E-4 0.14001202349879097 >1k 1.1423178830693511E-4 0.08076997839664411 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.2643394389736746E-5 0.0 0.0 0.0 6.793018316921024E-5 6 2.2643394389736746E-5 0.0 0.0 0.0 6.793018316921024E-5 7 2.2643394389736746E-5 0.0 0.0 0.0 6.793018316921024E-5 8 2.2643394389736746E-5 0.0 0.0 0.0 6.793018316921024E-5 9 2.2643394389736746E-5 0.0 0.0 0.0 6.793018316921024E-5 10 2.2643394389736746E-5 0.0 0.0 0.0 6.793018316921024E-5 11 2.2643394389736746E-5 0.0 0.0 0.0 6.793018316921024E-5 12 2.2643394389736746E-5 0.0 0.0 0.0 1.1321697194868372E-4 13 2.2643394389736746E-5 2.2643394389736746E-5 0.0 0.0 1.5850376072815722E-4 14 2.2643394389736746E-5 4.528678877947349E-5 0.0 0.0 1.8114715511789397E-4 15 2.2643394389736746E-5 1.5850376072815722E-4 0.0 0.0 2.0379054950763072E-4 16 2.2643394389736746E-5 1.5850376072815722E-4 0.0 0.0 2.2643394389736744E-4 17 2.2643394389736746E-5 1.5850376072815722E-4 0.0 0.0 2.2643394389736744E-4 18 2.2643394389736746E-5 1.5850376072815722E-4 0.0 2.2643394389736746E-5 2.2643394389736744E-4 19 2.2643394389736746E-5 1.5850376072815722E-4 0.0 2.2643394389736746E-5 2.2643394389736744E-4 20 2.2643394389736746E-5 1.5850376072815722E-4 0.0 4.528678877947349E-5 2.490773382871042E-4 21 2.2643394389736746E-5 1.5850376072815722E-4 0.0 4.528678877947349E-5 2.490773382871042E-4 22 2.2643394389736746E-5 1.5850376072815722E-4 0.0 1.1321697194868372E-4 2.490773382871042E-4 23 2.2643394389736746E-5 1.5850376072815722E-4 0.0 1.3586036633842047E-4 2.490773382871042E-4 24 2.2643394389736746E-5 1.8114715511789397E-4 0.0 1.8114715511789397E-4 2.490773382871042E-4 25 2.2643394389736746E-5 2.0379054950763072E-4 0.0 2.0379054950763072E-4 2.490773382871042E-4 26 2.2643394389736746E-5 2.0379054950763072E-4 0.0 3.8493770462552467E-4 2.490773382871042E-4 27 2.2643394389736746E-5 2.0379054950763072E-4 0.0 6.793018316921023E-4 2.490773382871042E-4 28 2.2643394389736746E-5 2.0379054950763072E-4 0.0 0.001335960268994468 2.490773382871042E-4 29 2.2643394389736746E-5 2.0379054950763072E-4 0.0 0.002309626227753148 2.490773382871042E-4 30 2.2643394389736746E-5 2.2643394389736744E-4 0.0 0.003939950623814194 2.490773382871042E-4 31 2.2643394389736746E-5 2.2643394389736744E-4 0.0 0.00876299362882812 2.490773382871042E-4 32 2.2643394389736746E-5 2.2643394389736744E-4 0.0 0.015329578001851777 2.490773382871042E-4 33 2.2643394389736746E-5 2.2643394389736744E-4 0.0 0.02239431705144964 2.490773382871042E-4 34 2.2643394389736746E-5 2.2643394389736744E-4 0.0 0.03262913131561065 2.490773382871042E-4 35 2.2643394389736746E-5 2.2643394389736744E-4 0.0 0.04494713786362744 2.490773382871042E-4 36 2.2643394389736746E-5 2.2643394389736744E-4 0.0 0.06154474595130448 2.490773382871042E-4 37 2.2643394389736746E-5 2.9436412706657767E-4 0.0 0.08215023484596491 2.490773382871042E-4 38 2.2643394389736746E-5 2.9436412706657767E-4 0.0 0.10483891602448113 2.7172073267684094E-4 39 2.2643394389736746E-5 3.3965091584605117E-4 0.0 0.12770874435811524 2.9436412706657767E-4 40 2.2643394389736746E-5 3.3965091584605117E-4 0.0 0.1499898444376162 2.9436412706657767E-4 41 2.2643394389736746E-5 3.3965091584605117E-4 0.0 0.17252002185540427 2.9436412706657767E-4 42 2.2643394389736746E-5 3.6229431023578794E-4 0.0 0.19543513697781786 2.9436412706657767E-4 43 2.2643394389736746E-5 3.6229431023578794E-4 0.0 0.22199583859697905 2.9436412706657767E-4 44 2.2643394389736746E-5 3.6229431023578794E-4 0.0 0.252360630473616 2.9436412706657767E-4 45 2.2643394389736746E-5 3.6229431023578794E-4 0.0 0.2865295126077288 2.9436412706657767E-4 46 2.2643394389736746E-5 4.0758109901526144E-4 0.0 0.32484213591516337 2.9436412706657767E-4 47 2.2643394389736746E-5 4.7551128218447167E-4 0.0 0.36317740261698767 2.9436412706657767E-4 48 2.2643394389736746E-5 4.7551128218447167E-4 0.0 0.40316563710926273 2.9436412706657767E-4 49 2.2643394389736746E-5 5.207980709639452E-4 0.0 0.44451247526492205 2.9436412706657767E-4 50 2.2643394389736746E-5 5.207980709639452E-4 0.0 0.484681856912315 2.9436412706657767E-4 51 2.2643394389736746E-5 5.660848597434186E-4 0.0 0.5259381214904154 2.9436412706657767E-4 52 2.2643394389736746E-5 5.660848597434186E-4 0.0 0.5674661068011926 3.3965091584605117E-4 53 2.2643394389736746E-5 5.660848597434186E-4 0.0 0.608722371379293 3.3965091584605117E-4 54 2.2643394389736746E-5 5.660848597434186E-4 0.0 0.6548922525399662 3.3965091584605117E-4 55 2.2643394389736746E-5 5.660848597434186E-4 0.0 0.7071532067914785 3.3965091584605117E-4 56 2.2643394389736746E-5 5.660848597434186E-4 0.0 0.7636711191882615 3.3965091584605117E-4 57 2.2643394389736746E-5 5.660848597434186E-4 0.0 0.824695067068602 3.6229431023578794E-4 58 2.2643394389736746E-5 5.660848597434186E-4 0.0 0.8871229054011062 3.6229431023578794E-4 59 2.2643394389736746E-5 5.660848597434186E-4 0.0 0.9533321905966965 3.6229431023578794E-4 60 2.2643394389736746E-5 5.887282541331553E-4 0.0 1.021488807709804 3.6229431023578794E-4 61 2.2643394389736746E-5 6.340150429126289E-4 0.0 1.0921361982057827 3.6229431023578794E-4 62 2.2643394389736746E-5 6.340150429126289E-4 0.0 1.1612891246720387 3.8493770462552467E-4 63 2.2643394389736746E-5 6.340150429126289E-4 0.0 1.2323440962670327 3.8493770462552467E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3910 0.0 35.21696 1 GTATCAA 7045 0.0 31.724669 1 TCAACGC 9650 0.0 22.612333 4 ATCAACG 9695 0.0 22.435272 3 CAACGCA 9730 0.0 22.323137 5 TATCAAC 10050 0.0 21.913818 2 AACGCAG 10145 0.0 21.405357 6 GTACATG 9490 0.0 19.249226 1 CGTGCCG 485 0.0 18.508669 10 TACATGG 9715 0.0 18.043047 2 ACGCAGA 12025 0.0 17.827726 7 CGCAGAG 12510 0.0 17.165325 8 ACATGGG 10150 0.0 16.864275 3 CCGGTAT 390 0.0 16.800447 58 AGAGTAC 12430 0.0 16.304615 11 GAGTACT 8565 0.0 16.164436 12 TCGCACG 195 8.997864E-6 15.922342 22 GGACCGT 460 0.0 15.761059 6 CAGAGTA 13225 0.0 15.663873 10 GACCGTG 800 0.0 15.534667 7 >>END_MODULE