FastQCFastQC Report
Mon 6 Feb 2017
SRR2077535_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077535_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4162643
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115300.026.7416061
GGTATCA70350.024.0490171
GTACATG162700.022.7495371
TACATGG164800.021.9385782
ATCAACG141050.021.3286553
TATCAAC144700.021.0521562
TCAACGC142850.020.9882074
CAACGCA145050.020.5521815
ACATGGG168750.020.5262683
AACGCAG149750.019.9587156
GAGTACT75950.018.9777112
ACGCCCG3950.018.33761821
AGAGTAC148900.018.10953711
CATGGGG80450.017.9261074
GTGTAGC25350.017.1616061
ACGCAGA173000.017.0985017
AGTACTT83350.017.08741413
CGCAGAG173200.016.9010338
GTACTTT84000.016.75012214
CATGGGA86300.016.5910194
Produced by FastQC (version 0.11.5)