FastQCFastQC Report
Mon 6 Feb 2017
SRR2077535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4162643
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA140350.029.286481
GGTATCA79750.026.3776511
ATCAACG180500.022.4364643
TATCAAC183950.022.2200662
TCAACGC184300.021.9178284
CAACGCA187450.021.4805165
AACGCAG194350.020.713166
GTACATG152150.020.2841171
TACATGG154300.019.5872382
TAGGGCG2850.019.36236631
AGAGTAC199650.019.02587111
GAGTACT128750.018.962412
ACATGGG157700.018.487183
TACACCG3001.8189894E-1218.4174865
AGTACTT135450.018.06650713
ACGCAGA223600.017.866567
CGCAGAG224750.017.6987488
GTACTTT137900.017.68675214
CGACGTT1200.001468524717.26597812
ACCGTCG1602.9317896E-517.264738
Produced by FastQC (version 0.11.5)