FastQCFastQC Report
Mon 6 Feb 2017
SRR2077534_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077534_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3061757
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45750.036.1406361
GTATCAA89450.034.0746771
TATCAAC117000.025.6187692
TCAACGC115900.025.5068744
ATCAACG115850.025.4876883
CAACGCA118750.024.8353945
AACGCAG122600.024.0393586
GTGGTAT22450.021.372231
GTACATG139750.021.1433261
ACGCAGA142450.020.6206597
TACATGG141100.020.4363612
CGCAGAG143450.020.3584048
AGAGTAC138300.019.7961411
TGGTATC23200.019.4763532
ACATGGG142750.019.3073833
GAGTACT76450.019.1684312
CAGAGTA149500.018.70487410
GCAGAGT152900.018.4915949
AGTACTT81150.018.10221313
GTACTTT81300.017.81286814
Produced by FastQC (version 0.11.5)