##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077534_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3061757
Sequences flagged as poor quality	0
Sequence length	75
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.922996501681876	31.0	30.0	34.0	26.0	34.0
2	29.926757414125287	31.0	30.0	34.0	25.0	34.0
3	30.050889407617912	31.0	31.0	34.0	26.0	34.0
4	33.43482386093998	37.0	35.0	37.0	27.0	37.0
5	33.52370844583682	37.0	35.0	37.0	28.0	37.0
6	33.53838335308779	37.0	35.0	37.0	28.0	37.0
7	33.43648663169546	37.0	35.0	37.0	27.0	37.0
8	33.358788434222575	37.0	35.0	37.0	27.0	37.0
9	34.74100034718627	39.0	35.0	39.0	27.0	39.0
10	34.66493552558221	39.0	35.0	39.0	27.0	39.0
11	34.68787431530327	39.0	35.0	39.0	27.0	39.0
12	34.60783432519302	39.0	35.0	39.0	26.0	39.0
13	34.5611768014248	39.0	35.0	39.0	25.0	39.0
14	35.68056805291864	40.0	36.0	41.0	25.0	41.0
15	35.70858954515332	40.0	36.0	41.0	25.0	41.0
16	35.69334405049127	40.0	36.0	41.0	25.0	41.0
17	35.633694313428535	40.0	36.0	41.0	25.0	41.0
18	35.600512385535495	40.0	36.0	41.0	25.0	41.0
19	35.70609881842354	40.0	36.0	41.0	25.0	41.0
20	35.704062732607454	40.0	36.0	41.0	25.0	41.0
21	35.68154984213313	40.0	36.0	41.0	25.0	41.0
22	35.6214657139675	40.0	36.0	41.0	25.0	41.0
23	35.58206186839779	40.0	36.0	41.0	25.0	41.0
24	35.56204362397146	40.0	36.0	41.0	25.0	41.0
25	35.600455228811434	40.0	36.0	41.0	25.0	41.0
26	35.54797196511676	40.0	36.0	41.0	25.0	41.0
27	35.55708242032271	40.0	36.0	41.0	25.0	41.0
28	35.52035220299978	40.0	36.0	41.0	25.0	41.0
29	35.49816788203636	40.0	36.0	41.0	25.0	41.0
30	35.406163193225325	40.0	36.0	41.0	25.0	41.0
31	35.38830906567699	40.0	35.0	41.0	25.0	41.0
32	35.31352390147226	40.0	35.0	41.0	25.0	41.0
33	35.20354587251699	40.0	35.0	41.0	24.0	41.0
34	35.166347296666586	40.0	35.0	41.0	24.0	41.0
35	35.11854108604961	40.0	35.0	41.0	24.0	41.0
36	35.04633973238242	40.0	35.0	41.0	24.0	41.0
37	34.97410473790049	40.0	35.0	41.0	24.0	41.0
38	34.88944681109572	40.0	35.0	41.0	23.0	41.0
39	34.852446487425354	40.0	35.0	41.0	23.0	41.0
40	34.80975857979585	40.0	35.0	41.0	22.0	41.0
41	34.73235171831076	40.0	35.0	41.0	22.0	41.0
42	34.63451867669446	40.0	35.0	41.0	21.0	41.0
43	34.524769601245296	39.0	35.0	41.0	19.0	41.0
44	34.42172484622392	39.0	34.0	41.0	18.0	41.0
45	34.309849867249426	39.0	34.0	41.0	17.0	41.0
46	34.20058874691885	39.0	34.0	41.0	15.0	41.0
47	34.078143366700886	39.0	34.0	41.0	14.0	41.0
48	33.954998714790236	39.0	33.0	41.0	9.0	41.0
49	33.850984255118874	39.0	33.0	41.0	9.0	41.0
50	33.730984856081	39.0	33.0	41.0	8.0	41.0
51	33.63814959841686	39.0	33.0	41.0	8.0	41.0
52	33.54623015477714	39.0	33.0	41.0	7.0	41.0
53	33.470885507896284	39.0	33.0	40.0	5.0	41.0
54	33.477482700292676	39.0	33.0	41.0	2.0	41.0
55	33.397654026756534	39.0	33.0	41.0	2.0	41.0
56	33.276017005921766	38.0	33.0	41.0	2.0	41.0
57	33.18968814311521	38.0	33.0	40.0	2.0	41.0
58	33.10638368753627	38.0	33.0	40.0	2.0	41.0
59	33.00523065677648	38.0	33.0	40.0	2.0	41.0
60	32.991353330783596	38.0	33.0	40.0	2.0	41.0
61	32.89810197216827	38.0	33.0	40.0	2.0	41.0
62	32.757966749157426	38.0	32.0	40.0	2.0	41.0
63	32.57387277958375	37.0	32.0	40.0	2.0	41.0
64	32.44914113040323	37.0	32.0	40.0	2.0	41.0
65	32.30480178538009	37.0	32.0	40.0	2.0	41.0
66	32.151767759492344	37.0	32.0	40.0	2.0	41.0
67	31.989631770254793	36.0	32.0	40.0	2.0	41.0
68	31.807021589237813	36.0	32.0	39.0	2.0	41.0
69	31.82958347118991	36.0	32.0	39.0	2.0	41.0
70	31.729039894413567	36.0	32.0	39.0	2.0	41.0
71	31.56376420467072	36.0	32.0	39.0	2.0	41.0
72	31.365036154077544	36.0	32.0	39.0	2.0	41.0
73	31.152650585921744	35.0	32.0	39.0	2.0	41.0
74	30.93545862718694	35.0	32.0	39.0	2.0	40.0
75	30.714902260368802	35.0	31.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7440092023423688
1101	2	0.747570529160452
1101	3	0.702387913402454
1101	4	0.8526135204496583
1101	5	0.8112222546482499
1101	6	0.7767439151392992
1101	7	0.7575863949091044
1101	8	0.7040776481515181
1101	9	0.8853767805730541
1101	10	0.9490009265970016
1101	11	0.8488246959735264
1101	12	0.7184403511558486
1101	13	0.8025889398480075
1101	14	0.9550033748508469
1101	15	0.9964653990503081
1101	16	0.9460431048000615
1101	17	0.919860320336177
1101	18	0.929710945355545
1101	19	0.98951247867722
1101	20	1.012029915374974
1101	21	0.9994417218845442
1101	22	1.023785456199633
1101	23	1.0407265087886088
1101	24	1.024561830377138
1101	25	0.8864958085618966
1101	26	0.8980921653234475
1101	27	0.8926642094063766
1101	28	0.9346939089751984
1101	29	0.927439081591622
1101	30	0.962464975355168
1101	31	1.0476262214265688
1101	32	0.9854243970538761
1101	33	1.0640684398274516
1101	34	1.0776001485570532
1101	35	1.08768334370302
1101	36	1.065737789772676
1101	37	1.1126173265855783
1101	38	1.0414714474689575
1101	39	1.0899563841026563
1101	40	1.0438317079877777
1101	41	1.1858871150025792
1101	42	1.03843001490538
1101	43	0.9464033095081561
1101	44	0.9554285238700899
1101	45	1.0613897407066162
1101	46	1.065330726644767
1101	47	1.0987047073342495
1101	48	1.0403694963590056
1101	49	1.0183276809524955
1101	50	0.9812733528995707
1101	51	1.027796642815403
1101	52	1.0080393701012866
1101	53	1.0354705466469767
1101	54	0.9688918871395629
1101	55	0.8887182013071069
1101	56	0.9443419980589525
1101	57	0.8730800498305129
1101	58	0.8867881556601347
1101	59	0.859986430920415
1101	60	0.9235013351988002
1101	61	0.8716103397096404
1101	62	0.8435362638018873
1101	63	0.9165497748808065
1101	64	0.9236099498553614
1101	65	0.8297388947637501
1101	66	0.8257445606170748
1101	67	0.851687446221483
1101	68	0.8725654188791196
1101	69	0.8945744091030434
1101	70	0.8923044630658019
1101	71	0.8615870446879548
1101	72	0.9434743974743505
1101	73	0.9500767161137453
1101	74	0.8924050842013109
1101	75	0.9407401472514323
1102	1	0.970879993350998
1102	2	0.9852379459656255
1102	3	1.0727727202580581
1102	4	1.0446789644142171
1102	5	1.0584107881777527
1102	6	1.034282274696423
1102	7	1.0320799626784662
1102	8	1.054308990156997
1102	9	1.3145670036889712
1102	10	1.2907706929389136
1102	11	1.2750764452367704
1102	12	1.2363967546794896
1102	13	1.2383403976043539
1102	14	1.4578342529299704
1102	15	1.4387950627391959
1102	16	1.453714572530643
1102	17	1.3929967615382992
1102	18	1.4443753619024875
1102	19	1.4192599813460376
1102	20	1.4059205164293687
1102	21	1.4362067983818108
1102	22	1.51163256903898
1102	23	1.5357285187220242
1102	24	1.4509048696626863
1102	25	1.4842919365338645
1102	26	1.5249341605972262
1102	27	1.4523273969211843
1102	28	1.4233697311178304
1102	29	1.4382793003149743
1102	30	1.4740148824900317
1102	31	1.4701898759735883
1102	32	1.5258455779363445
1102	33	1.6192538683869628
1102	34	1.5749989930006336
1102	35	1.5590333622937962
1102	36	1.510353364493838
1102	37	1.6277029195780557
1102	38	1.717046452593621
1102	39	1.6784524658417226
1102	40	1.6626250957061472
1102	41	1.6367278262261706
1102	42	1.6381353577787223
1102	43	1.5684484189807577
1102	44	1.6284823492658873
1102	45	1.6253284736765323
1102	46	1.6193377041752228
1102	47	1.610750886109173
1102	48	1.6865636452786532
1102	49	1.6902069376409585
1102	50	1.557566631912934
1102	51	1.5545480579771294
1102	52	1.5972776408308889
1102	53	1.6203481936139283
1102	54	1.6067346074091873
1102	55	1.5460832318376845
1102	56	1.5950769104693308
1102	57	1.5451382260888025
1102	58	1.5731188558179952
1102	59	1.5420974910560261
1102	60	1.6385338180300622
1102	61	1.626532569040826
1102	62	1.6441386670255866
1102	63	1.5643189262629562
1102	64	1.5263746731722776
1102	65	1.5507228658775034
1102	66	1.491069903614651
1102	67	1.5169097413081047
1102	68	1.5626730634589947
1102	69	1.6012307273487139
1102	70	1.6182060047246658
1102	71	1.583660284354547
1102	72	1.56969344649632
1102	73	1.606825123030756
1102	74	1.6463146931028732
1102	75	1.6197520054149095
1103	1	0.7574149589402737
1103	2	0.8353313190198897
1103	3	0.8431750829340992
1103	4	0.9207446658321032
1103	5	0.8871381079057485
1103	6	0.9040398833088759
1103	7	0.8380722665385818
1103	8	0.8069467344965773
1103	9	0.978394623373589
1103	10	0.9331999463016629
1103	11	0.8748289109588541
1103	12	0.8271278294076296
1103	13	0.8822042852279139
1103	14	1.0041189590741268
1103	15	1.0014661176988966
1103	16	1.0699177516274645
1103	17	1.0084631729636726
1103	18	1.0409048938890066
1103	19	1.109437437206907
1103	20	1.0253742364812908
1103	21	0.962250706827902
1103	22	1.0020677654831758
1103	23	1.079247431131428
1103	24	1.0607492904318505
1103	25	0.9662457036435441
1103	26	1.0270847525420237
1103	27	1.1124617666964696
1103	28	1.0989666012957215
1103	29	1.0256637316836361
1103	30	0.9970392512990642
1103	31	0.9975778063481826
1103	32	1.0685496590781511
1103	33	1.136705343469984
1103	34	1.0566982299119871
1103	35	1.089360624966865
1103	36	1.0944451120684775
1103	37	1.1052703805378883
1103	38	1.065701583625433
1103	39	1.0842037189376015
1103	40	1.1117662706610503
1103	41	1.0855520933067808
1103	42	1.1919795794412735
1103	43	1.1556602529408977
1103	44	1.1480523647301126
1103	45	1.03716199461347
1103	46	1.2121138046052664
1103	47	1.0836398122259823
1103	48	1.2138575124184925
1103	49	1.1763406887635455
1103	50	1.1137050239764434
1103	51	1.1246642258106192
1103	52	1.1219616845690936
1103	53	1.1470603222602236
1103	54	1.2271880957277759
1103	55	1.1843386709827968
1103	56	1.1216310296128924
1103	57	1.07776290091968
1103	58	1.1357846821376327
1103	59	1.14495216121459
1103	60	1.1482046061199753
1103	61	1.1807045741489972
1103	62	1.1381797950900392
1103	63	1.2121865404428576
1103	64	1.2205093206929192
1103	65	1.127399826690727
1103	66	1.1344153192894453
1103	67	1.0746427117319897
1103	68	1.1195402233746492
1103	69	1.1325065176158304
1103	70	1.1622451145340555
1103	71	1.1683343455731325
1103	72	1.1374038647498743
1103	73	1.1215444768210396
1103	74	1.2166125038921933
1103	75	1.1738596459638941
1104	1	0.46030055437710615
1104	2	0.4908855204996705
1104	3	0.45801657287514175
1104	4	0.508294883431077
1104	5	0.4413698170526672
1104	6	0.4843882665199146
1104	7	0.476480221189135
1104	8	0.5312406676724919
1104	9	0.5033317275531317
1104	10	0.5722385205228235
1104	11	0.4979661386440952
1104	12	0.5033105009251813
1104	13	0.5230480772976378
1104	14	0.6692543209328861
1104	15	0.6879054885063525
1104	16	0.7293263722734409
1104	17	0.6857716760448938
1104	18	0.639178242414971
1104	19	0.5611071000785088
1104	20	0.6182580136591724
1104	21	0.6037842281229402
1104	22	0.626394481283306
1104	23	0.6101908357106325
1104	24	0.673367198078985
1104	25	0.6074409058582759
1104	26	0.6496219397848719
1104	27	0.6367042449929059
1104	28	0.5428269444185219
1104	29	0.6178862102309068
1104	30	0.6402310907090722
1104	31	0.6672811003648533
1104	32	0.6784932920514635
1104	33	0.7750174135665375
1104	34	0.6805736037755565
1104	35	0.6423080606946101
1104	36	0.5747694666802374
1104	37	0.6180520314220814
1104	38	0.5744176594678692
1104	39	0.5823974268222827
1104	40	0.5684862864142985
1104	41	0.635323546801196
1104	42	0.5718299625622763
1104	43	0.6058185973629122
1104	44	0.5037476349234424
1104	45	0.5317492640727011
1104	46	0.5400197923733145
1104	47	0.5682116677060236
1104	48	0.5265046151385278
1104	49	0.5222738720692846
1104	50	0.5032055703784977
1104	51	0.6164082859024091
1104	52	0.5590195207430071
1104	53	0.6330859354779221
1104	54	0.6489110986921034
1104	55	0.534426018863499
1104	56	0.5699770610291495
1104	57	0.48721203141565894
1104	58	0.4225664392493158
1104	59	0.5657156690822944
1104	60	0.5969756974116152
1104	61	0.6459849969963827
1104	62	0.6235623971949735
1104	63	0.7279950753273781
1104	64	0.6629222517468278
1104	65	0.6864305602635241
1104	66	0.6338654695893595
1104	67	0.5535018164500585
1104	68	0.5820184588550248
1104	69	0.6127953964415909
1104	70	0.5934155191743677
1104	71	0.5643915667168464
1104	72	0.6588943714045605
1104	73	0.7028643469436879
1104	74	0.697412383934072
1104	75	0.6747357229975144
1105	1	0.527144605190891
1105	2	0.4002850818957775
1105	3	0.4818510128589715
1105	4	0.5441312504769868
1105	5	0.4162037362924167
1105	6	0.5083414960482671
1105	7	0.558590938843885
1105	8	0.5293069507172206
1105	9	0.6107450935397267
1105	10	0.6405303872418102
1105	11	0.5511976396918854
1105	12	0.5754466045568236
1105	13	0.5082190161176925
1105	14	0.5678217874760918
1105	15	0.640267886457984
1105	16	0.6090005019879143
1105	17	0.6525728266730297
1105	18	0.7011829621484154
1105	19	0.6603568815965417
1105	20	0.705817649076522
1105	21	0.6606012339942069
1105	22	0.718545125819972
1105	23	0.7433931313577986
1105	24	0.7277098733535752
1105	25	0.6980392529295685
1105	26	0.7812728886632954
1105	27	0.7736546145881036
1105	28	0.8000352570246889
1105	29	0.7759048253463661
1105	30	0.7723471679160951
1105	31	0.7292256885361397
1105	32	0.7532042149140779
1105	33	0.8261404596175694
1105	34	0.7635660925591168
1105	35	0.8217680222246742
1105	36	0.7906030814593876
1105	37	0.7179677283979871
1105	38	0.7037960631103672
1105	39	0.7289207411123684
1105	40	0.7206274097241661
1105	41	0.6641503324887452
1105	42	0.6887314711039281
1105	43	0.7309481951398098
1105	44	0.7709104067840613
1105	45	0.5907949880650278
1105	46	0.6219102336177684
1105	47	0.6679190195144002
1105	48	0.7054930786725393
1105	49	0.7193043711303488
1105	50	0.7715636055832675
1105	51	0.713764137119334
1105	52	0.703584732709146
1105	53	0.695500405460983
1105	54	0.6595753480541262
1105	55	0.6657934224705855
1105	56	0.6627453731694715
1105	57	0.6129528320590865
1105	58	0.6302882825265002
1105	59	0.6491857878552025
1105	60	0.6646532714999935
1105	61	0.740191095192408
1105	62	0.7134088858326066
1105	63	0.7842383833143458
1105	64	0.7703704132302533
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2204	1	0.7088085791872061
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2211	60	-0.40328145054943576
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2211	75	-0.3230758120457722
2212	1	-0.4083604301476953
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2212	3	-0.3397625231542918
2212	4	-0.31022031397724703
2212	5	-0.460941734222466
2212	6	-0.39403602467727694
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2212	13	-0.5167098124835263
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2212	51	-0.46697433183931736
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2212	54	-0.5713825125011311
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2212	59	-0.3086491471611268
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2212	61	-0.3354693626408718
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2212	64	-0.3487257147071148
2212	65	-0.30126126153382415
2212	66	-0.25544168486070973
2212	67	-0.32421168191391914
2212	68	-0.33705815001246364
2212	69	-1.0680632937680876
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2212	71	-0.5576112093273906
2212	72	-0.4670896503737687
2212	73	-0.45418854873981473
2212	74	-0.44050045791315284
2212	75	-0.4748115827260051
2213	1	-0.25266432390136373
2213	2	-0.24727449399647128
2213	3	-0.2731535299536283
2213	4	-0.20757959582906693
2213	5	-0.3162606923308715
2213	6	-0.1336867596166158
2213	7	-0.11806173218304394
2213	8	-0.22327007251302433
2213	9	-0.2384794808732238
2213	10	-0.31389334701309224
2213	11	-0.3625678168870081
2213	12	-0.3553767461294228
2213	13	-0.42005112700713454
2213	14	-0.5993862397681937
2213	15	-0.35939785485979314
2213	16	-0.41970084113187767
2213	17	-0.4145919756569185
2213	18	-0.4939783654020147
2213	19	-0.5431071966111745
2213	20	-0.3946588741295898
2213	21	-0.4027461369447991
2213	22	-0.32805217115407714
2213	23	-0.3823387021129747
2213	24	-0.30031120193960703
2213	25	-0.3688741734422436
2213	26	-0.23567295639426789
2213	27	-0.4805576113481038
2213	28	-0.49318451648515804
2213	29	-0.3713221705011094
2213	30	-0.48289825937057174
2213	31	-0.285494493092429
2213	32	-0.2803603507279888
2213	33	-0.31435645903356146
2213	34	-0.40332844914138377
2213	35	-0.4622014097873617
2213	36	-0.5730445557534836
2213	37	-0.3747385918818438
2213	38	-0.3553824117216138
2213	39	-0.400683708955512
2213	40	-0.4403730162300903
2213	41	-0.37644772406874694
2213	42	-0.34773965528278694
2213	43	-0.42058387627782423
2213	44	-0.47531800961035486
2213	45	-0.3525121795070518
2213	46	-0.27856083401663057
2213	47	-0.3120875691375886
2213	48	-0.3199172957265617
2213	49	-0.3495816640748757
2213	50	-0.3157542884821183
2213	51	-0.3348991428962762
2213	52	-0.32722980513049293
2213	53	-0.3609366445854931
2213	54	-0.3481703047994529
2213	55	-0.2816387868051464
2213	56	-0.24544574809739572
2213	57	-0.3454748721733836
2213	58	-0.28964259957649574
2213	59	-0.35848117886791186
2213	60	-0.39158926643196423
2213	61	-0.3345739480220118
2213	62	-0.33751090257513994
2213	63	-0.17573324771274912
2213	64	-0.20369715612370243
2213	65	-0.2369427450588546
2213	66	-0.36072925180110005
2213	67	-0.31388767737724166
2213	68	-0.21017277829675862
2213	69	-0.34838778648567725
2213	70	-0.38458456432477917
2213	71	-0.3071177492486683
2213	72	-0.26950648648462305
2213	73	-0.39874232300268986
2213	74	-0.3751404131367444
2213	75	-0.3023062723394432
2214	1	-0.8264521238258418
2214	2	-0.7381836312957972
2214	3	-0.6614378679072956
2214	4	-0.7020066174261075
2214	5	-0.660038038546908
2214	6	-0.649401366086245
2214	7	-0.6949565579618593
2214	8	-0.6687315301128152
2214	9	-0.8443702257692038
2214	10	-0.7870487486060824
2214	11	-0.9212169848913732
2214	12	-0.870967132799997
2214	13	-0.9145108073306858
2214	14	-0.9679678158725054
2214	15	-1.0469544426643793
2214	16	-1.0866252784613835
2214	17	-0.9898206960909306
2214	18	-1.2607782913366847
2214	19	-1.1748923750896623
2214	20	-1.0950199425948242
2214	21	-0.9536117389953063
2214	22	-0.9416481156400707
2214	23	-1.0391535869107713
2214	24	-0.9407080054667531
2214	25	-1.034682455053776
2214	26	-0.9526757600876223
2214	27	-1.0005060426357417
2214	28	-1.020125997587563
2214	29	-1.0074376190993348
2214	30	-0.986344994299813
2214	31	-1.0847144744011388
2214	32	-1.112107767553944
2214	33	-1.0574447644393103
2214	34	-1.0248873638802394
2214	35	-1.1147144550532317
2214	36	-1.1078864598155604
2214	37	-1.0150370547347265
2214	38	-1.0495209722007601
2214	39	-1.0576919217504397
2214	40	-1.0283922936266023
2214	41	-0.984283777261652
2214	42	-0.9692615373585411
2214	43	-0.86792940706232
2214	44	-0.8552694151748739
2214	45	-1.0060831342353538
2214	46	-1.0674484836234868
2214	47	-0.9938479619754119
2214	48	-0.9805835045878624
2214	49	-0.9664017830167921
2214	50	-0.9912406337810822
2214	51	-1.0046787402316752
2214	52	-0.8801086572928938
2214	53	-0.9255461030775649
2214	54	-0.8410827050554701
2214	55	-0.9508760889027386
2214	56	-0.8802584196832797
2214	57	-1.019059940137069
2214	58	-0.9461536415794072
2214	59	-0.9257222892062913
2214	60	-0.9862043673022356
2214	61	-1.0864302467093943
2214	62	-0.9721066998052734
2214	63	-0.9710901682281197
2214	64	-0.9072728492833022
2214	65	-0.9431740009181091
2214	66	-0.9073572382885509
2214	67	-0.918332690433445
2214	68	-0.8840772894291575
2214	69	-0.8384203868930378
2214	70	-0.8732481225805131
2214	71	-0.8398991646837075
2214	72	-0.8922732638118873
2214	73	-0.9515396741466908
2214	74	-0.9462926975709252
2214	75	-0.9315875179199473
2215	1	-1.2107562702727144
2215	2	-1.211513734725088
2215	3	-1.359911688910092
2215	4	-1.4559382526587683
2215	5	-1.2540701823414437
2215	6	-1.1696693428704066
2215	7	-1.201394000114675
2215	8	-1.2908460779032254
2215	9	-1.3907836508554894
2215	10	-1.2983981446271784
2215	11	-1.3265928016591104
2215	12	-1.3262755557450774
2215	13	-1.5019570057467817
2215	14	-1.634581527900579
2215	15	-1.5773536943265327
2215	16	-1.6566191409858817
2215	17	-1.9795927214381095
2215	18	-1.8179762081472504
2215	19	-1.6710327713219186
2215	20	-1.6438863844180673
2215	21	-1.8731856482119724
2215	22	-1.693784884644046
2215	23	-1.8084096696226268
2215	24	-1.6792367479947217
2215	25	-1.7988519322632186
2215	26	-1.6963591926533397
2215	27	-1.768563245486213
2215	28	-1.8107103010432937
2215	29	-1.714377110695331
2215	30	-1.7202721102980973
2215	31	-1.754669262322743
2215	32	-1.8015243718920146
2215	33	-1.793464739849938
2215	34	-1.8395864203667216
2215	35	-1.7467560241156193
2215	36	-1.7763049053962803
2215	37	-1.906946934491522
2215	38	-1.6880113308385205
2215	39	-1.6949824137944134
2215	40	-1.7033295867828286
2215	41	-1.8320497164434144
2215	42	-1.8169559384415734
2215	43	-1.8051209298469573
2215	44	-1.7572962621899109
2215	45	-1.6616414964963866
2215	46	-1.6526473990072361
2215	47	-1.7483390744767249
2215	48	-1.8409292950887917
2215	49	-1.7280338778694002
2215	50	-1.7304053253384026
2215	51	-1.648947897147778
2215	52	-1.6135182321493744
2215	53	-1.6282673136798032
2215	54	-1.7385893309635136
2215	55	-1.7717471565274572
2215	56	-1.6052082443409361
2215	57	-1.6131488210443905
2215	58	-1.6268060887744262
2215	59	-1.6818839396543694
2215	60	-1.7084421579689284
2215	61	-1.7512160369735028
2215	62	-1.9419590649041538
2215	63	-1.6915212518773721
2215	64	-1.7305796658280421
2215	65	-1.7158940077068294
2215	66	-1.8298281938204681
2215	67	-1.779815690020989
2215	68	-1.8160657722886278
2215	69	-1.7670830882111126
2215	70	-1.741901706491248
2215	71	-1.7812472976873401
2215	72	-1.815879454965632
2215	73	-1.718409344558335
2215	74	-1.6714433222712053
2215	75	-1.7039735157925513
2216	1	-1.3827471412949244
2216	2	-1.1791048308736727
2216	3	-1.3748583246649133
2216	4	-1.2151273846313586
2216	5	-1.1775127570436439
2216	6	-1.3278887791250469
2216	7	-1.3164614605755602
2216	8	-1.4264599345349005
2216	9	-1.5764370078718457
2216	10	-1.649914907764284
2216	11	-1.5231549773299733
2216	12	-1.4494883569677839
2216	13	-1.636156077358656
2216	14	-1.9888999784528067
2216	15	-1.8993401764171054
2216	16	-1.7747913833168667
2216	17	-1.6820200588023724
2216	18	-1.9884603847578575
2216	19	-1.7751439093977126
2216	20	-1.7764192110449386
2216	21	-2.012411853748226
2216	22	-1.8928053783730334
2216	23	-1.9402258095314409
2216	24	-1.8284294427471082
2216	25	-1.8837767266288665
2216	26	-1.9747718451687177
2216	27	-2.0821952671313966
2216	28	-2.029081336159912
2216	29	-2.065516942057087
2216	30	-2.0646029014933873
2216	31	-1.8547250275717033
2216	32	-1.912483276001602
2216	33	-1.9206198343668746
2216	34	-1.9224401553475374
2216	35	-2.045530737673289
2216	36	-1.9868610604856372
2216	37	-2.101537705342814
2216	38	-2.1147595455760353
2216	39	-2.153313380546969
2216	40	-2.069468204734072
2216	41	-2.121216800488874
2216	42	-1.9417162253228
2216	43	-2.0272607663721587
2216	44	-2.0968627390137726
2216	45	-2.166792553196224
2216	46	-2.142537365909533
2216	47	-2.105368335742078
2216	48	-2.0863180769795413
2216	49	-2.1343560047973043
2216	50	-2.0912493427082453
2216	51	-2.3441025166274088
2216	52	-2.196091075505823
2216	53	-2.211055472858611
2216	54	-2.195413603304466
2216	55	-2.254021036188117
2216	56	-2.2968222868567736
2216	57	-2.211297724586
2216	58	-2.216617571252261
2216	59	-2.1280245197162273
2216	60	-2.2446709813738117
2216	61	-2.2633387824825917
2216	62	-2.3116124425743045
2216	63	-2.3350382835138213
2216	64	-2.4264458085767515
2216	65	-2.5509946175101526
2216	66	-2.4208422435651364
2216	67	-2.27250682799351
2216	68	-2.3645877350188513
2216	69	-2.179596706807793
2216	70	-2.1325625763258635
2216	71	-2.184418688049245
2216	72	-2.2116552373572382
2216	73	-2.219718278874101
2216	74	-2.300372061511869
2216	75	-2.2496411239280825
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	117696.0
3	18796.0
4	13025.0
5	9587.0
6	7784.0
7	6967.0
8	6505.0
9	6809.0
10	6988.0
11	7667.0
12	8079.0
13	8565.0
14	8846.0
15	9532.0
16	10025.0
17	10719.0
18	11435.0
19	12593.0
20	14514.0
21	17378.0
22	22607.0
23	27936.0
24	29367.0
25	29534.0
26	29907.0
27	30996.0
28	36479.0
29	43144.0
30	47705.0
31	52617.0
32	63752.0
33	79736.0
34	104530.0
35	139495.0
36	191959.0
37	293777.0
38	501760.0
39	843641.0
40	179305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.590746515790606	18.701344598861887	13.431786722863418	23.27612216248409
2	15.436797570000177	20.46266929777113	40.21766786208809	23.882865270140606
3	19.085217192239455	25.83169134419635	29.097464659760774	25.98562680380342
4	12.135347495273175	16.625079325617758	38.45853151107942	32.78104166802965
5	12.99453527602646	38.71226687584354	34.475552016936525	13.817645831193479
6	30.748084413354654	37.611525895984386	17.966878256006748	13.673511434654213
7	27.69744705830413	33.18166878962145	21.392385814192675	17.728498337881742
8	25.89356271278319	35.36154629073512	20.03886782422249	18.7060231722592
9	26.419778601261214	16.778514930615053	19.734847345268754	37.06685912285498
10	14.246551600096796	30.443664689300643	33.52887891848761	21.780904792114953
11	33.671859361569346	24.663207486691185	23.32676560130727	18.338167550432203
12	22.917267685995448	26.708644800356918	30.01943541143739	20.354652102210245
13	27.423578586072576	23.48087056113304	26.07990034130095	23.015650511493433
14	21.351506963141315	22.86736826953589	28.04135683931942	27.739767928003378
15	23.191409816054065	30.43519076086832	24.31902192063016	22.054377502447455
16	22.234771766208212	29.91591042307773	26.19736387615399	21.65195393456007
17	21.062527369848706	29.428094031598746	27.170381989061543	22.338996609491005
18	20.7568952486044	28.86475851217717	28.73622838985116	21.642117849367278
19	21.863120669703456	28.47976962012644	29.068184472718457	20.58892523745165
20	22.24889186332801	27.776900056555775	28.996888941661197	20.977319138455016
21	22.835967377329666	27.63796425638828	28.64530206721474	20.880766299067314
22	22.251790159601438	27.557008691556074	28.647067867412062	21.544133281430426
23	21.878847425441535	27.76451065247101	28.489965570910087	21.86667635117737
24	21.34552041137113	27.748676027569115	29.049602227616937	21.85620133344281
25	21.794898642988773	27.72419872466075	28.92280689814039	21.558095734210088
26	21.53317459413869	27.838640888887145	29.069556688409882	21.558627828564287
27	21.724768877083246	28.134466517852765	28.514681466909845	21.626083138154144
28	21.48433448259276	27.92095075831817	28.78716537785353	21.80754938123554
29	21.566271525631812	28.165218427000998	28.837573344537066	21.43093670283012
30	21.68819683491313	28.024456150992883	28.777267702064748	21.510079312029248
31	21.921277527245028	27.692518112634378	28.706668577565136	21.679535782555458
32	21.45778233769157	28.063513957393017	28.700433293594035	21.778270411321383
33	21.643584338788322	27.79613777018365	28.704499303374625	21.855778587653404
34	21.819146937795068	27.8268231493281	28.853437899461788	21.500592013415044
35	21.96215198107861	27.95908523919409	28.531923303966085	21.54683947576122
36	21.806129704764867	27.920094791475442	28.50753492097929	21.766240582780398
37	21.865513836482307	27.690572576034135	28.61773945920011	21.82617412828345
38	21.573751907907308	27.847036231258	28.97833036184133	21.600881498993356
39	21.79185928957726	28.18638372773999	28.499357105699673	21.522399876983076
40	21.94599915826183	27.73020000772357	28.93913499948619	21.384665834528413
41	21.78347033876119	27.95440860778051	28.571391172312687	21.69072988114561
42	21.738622228959706	27.84554369474658	28.463573223042328	21.952260853251385
43	21.432246459346306	27.730113033847314	28.676774778030012	22.16086572877637
44	21.59672714587984	28.161938476258914	28.511521548623886	21.729812829237357
45	22.000932568736044	27.926539760967906	28.21530885204061	21.85721881825544
46	21.91316250909542	27.816879856005137	28.176506959326165	22.09345067557328
47	21.822837750717504	27.988271215061538	28.1490511268993	22.039839907321653
48	21.476199626615877	28.078315731858737	28.379517494483203	22.065967147042176
49	21.839310005025347	28.020454965845644	28.083378212253006	22.056856816876003
50	21.981189638195797	28.05818956487126	28.052264680619277	21.908356116313662
51	21.854326750413566	28.390146933021576	27.721236815854272	22.034289500710585
52	21.905968668443208	28.287664529298333	27.79329512933375	22.01307167292471
53	21.910845120440424	28.2226138983944	27.86031889422943	22.006222086935743
54	21.780082506857575	28.186963183458314	28.083878699342158	21.949075610341957
55	21.86702709071035	28.14099081704116	27.797038937578133	22.194943154670348
56	21.818013393565863	28.574369838257912	27.75002954126104	21.857587226915182
57	22.00600002683551	28.13100833111613	27.61720653490432	22.245785107144037
58	21.679465776293824	28.005761236047004	28.172935284297356	22.141837703361812
59	21.961795608996248	28.221015557937456	27.840988332692753	21.976200500373544
60	21.731059599093268	28.446548606045685	27.883767952342726	21.938623842518325
61	22.149157695639108	28.072392804322593	27.923841360938106	21.854608139100193
62	21.74549466595295	28.301404351377585	27.98213090145968	21.97097008120978
63	21.724359469736164	28.062730772303784	28.068854138150996	22.144055619809055
64	21.96174618831996	27.85352593703767	28.077687524574458	22.107040350067916
65	21.667127856580308	28.05571610786277	27.94096987420158	22.336186161355343
66	21.637100702355326	28.278607492837654	27.842777943633497	22.241513861173527
67	21.838612298887455	28.16001257260351	27.669222659498537	22.332152469010495
68	21.81826990086027	28.096377686146003	28.085051920126507	22.000300492867222
69	21.839841554706798	28.270380831026053	27.515075181977405	22.37470243228974
70	21.79837859106464	27.88409603800353	27.901674504414913	22.41585086651692
71	21.818262161790603	27.990808691155	27.929336903366874	22.261592243687524
72	21.711081688398195	28.303729683462166	27.579494514882562	22.405694113257077
73	21.88665411995745	27.938073807380736	27.922690450863268	22.25258162179854
74	21.8864042658623	28.259635796638392	27.55129894485891	22.30266099264039
75	21.782488429173313	28.239594113373638	27.65932579001974	22.318591667433306
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4322.0
1	3490.5
2	3241.0
3	3728.5
4	3634.0
5	3153.5
6	2543.0
7	2506.5
8	2600.0
9	3292.5
10	4794.5
11	5521.0
12	5438.0
13	5214.5
14	5095.0
15	5536.5
16	5874.0
17	6284.5
18	7211.0
19	8568.5
20	9410.0
21	10669.0
22	13726.5
23	17990.5
24	20456.0
25	23653.5
26	30751.0
27	38893.0
28	43135.0
29	48659.5
30	59328.0
31	69726.5
32	74981.0
33	82889.0
34	96935.0
35	109546.5
36	116020.0
37	118257.0
38	122972.5
39	127891.5
40	130332.0
41	132508.5
42	135852.0
43	138561.0
44	140103.0
45	141095.0
46	142626.0
47	143597.5
48	144030.0
49	143552.0
50	138683.0
51	131135.5
52	127979.0
53	123298.0
54	113762.5
55	103764.0
56	98620.0
57	92800.5
58	80194.0
59	66864.0
60	60321.0
61	54397.0
62	42450.0
63	30623.5
64	24820.0
65	20589.5
66	13467.0
67	8903.5
68	7232.0
69	6135.0
70	4035.5
71	2484.5
72	1936.0
73	1815.0
74	1543.5
75	1052.5
76	712.0
77	583.0
78	374.5
79	236.0
80	177.0
81	167.5
82	116.0
83	59.5
84	45.0
85	42.5
86	40.5
87	29.0
88	17.0
89	14.5
90	10.0
91	10.5
92	13.0
93	9.5
94	7.0
95	8.5
96	9.0
97	6.5
98	5.0
99	9.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19031556064050806
2	0.1299580600289311
3	0.13005604298446938
4	0.15471508679493506
5	0.15334332541739923
6	0.1289129085031895
7	0.12776977402190964
8	0.10405789878164726
9	0.1223153894969457
10	0.12466698042986428
11	0.14321842001177754
12	0.1453740450336196
13	0.1608226910234875
14	0.16157389368261427
15	0.1469744333074114
16	0.1483135336997678
17	0.1340406831763592
18	0.1341713271170769
19	0.1711435623401857
20	0.15017520985499502
21	0.16288033308979125
22	0.1556949163503178
23	0.17408305100633395
24	0.15866706600164546
25	0.18384868557498193
26	0.1688899543628054
27	0.1826075681381638
28	0.18244426321226667
29	0.18397932951569965
30	0.1746709487395636
31	0.1829994999603169
32	0.16882463239244655
33	0.18273821207888152
34	0.19867677284644078
35	0.18763735985579522
36	0.18897646024815165
37	0.20641742633396445
38	0.19769694329105805
39	0.17202540894003018
40	0.20168158348294785
41	0.19325504930665627
42	0.24606786234178613
43	0.20867103431134476
44	0.19044620458122574
45	0.18557971778949148
46	0.21713022947281577
47	0.196847757676393
48	0.22843093034489673
49	0.2365308546693941
50	0.22356444355316243
51	0.23633488875831754
52	0.22059229390183477
53	0.21268833548841398
54	0.1963251819135222
55	0.19913402663895274
56	0.21908988858358125
57	0.19936265353520868
58	0.22395637537531554
59	0.2364328717138558
60	0.23780463309139166
61	0.2261120003971576
62	0.23914373348374804
63	0.2574012241990465
64	0.2372493963433414
65	0.26331286251652236
66	0.2251321708417748
67	0.2448594058901474
68	0.22131083557578213
69	0.2145826726288206
70	0.2248055609899806
71	0.21873061774660757
72	0.21791409311712195
73	0.21252503056251687
74	0.21004279568888057
75	0.2386864796912361
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	3061757.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.94920336312731
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8643293482654	40.587860546388484
2	12.534994209327035	13.775758919278688
3	5.275762148115424	8.696967815168515
4	2.7621672553218763	6.0711556094261185
5	1.6488048988891386	4.530025784758992
6	1.0091743469416423	3.3271995851368494
7	0.6876150121941248	2.6448727998415955
8	0.4563480407497403	2.0060769036417745
9	0.33895017372866115	1.6762537823565178
>10	1.3438146663601909	11.882936105703907
>50	0.050353254884548684	1.892986384923217
>100	0.02691076084665671	2.4718231986933183
>500	5.371507216661217E-4	0.1794573057764787
>1k	2.387336538932508E-4	0.2566252589054401
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	9.798295553827426E-5	0.0
9	0.0	0.0	0.0	1.3064394071769903E-4	0.0
10	0.0	0.0	0.0	1.6330492589712378E-4	0.0
11	0.0	0.0	0.0	1.6330492589712378E-4	0.0
12	0.0	0.0	0.0	1.9596591107654853E-4	0.0
13	0.0	0.0	0.0	1.9596591107654853E-4	0.0
14	0.0	0.0	0.0	1.9596591107654853E-4	0.0
15	0.0	0.0	0.0	1.9596591107654853E-4	0.0
16	0.0	0.0	0.0	2.6128788143539805E-4	0.0
17	0.0	0.0	0.0	2.939488666148228E-4	0.0
18	0.0	0.0	0.0	3.2660985179424755E-4	0.0
19	0.0	0.0	0.0	4.245928073325218E-4	0.0
20	0.0	0.0	0.0	4.245928073325218E-4	0.0
21	0.0	0.0	0.0	4.899147776913713E-4	0.0
22	0.0	0.0	0.0	5.878977332296456E-4	0.0
23	0.0	0.0	0.0	9.145075850238931E-4	0.0
24	0.0	0.0	0.0	0.0013717613775358398	0.0
25	0.0	0.0	0.0	0.001469744333074114	0.0
26	0.0	0.0	0.0	0.002024981081124335	0.0
27	0.0	0.0	0.0	0.0026455397995334053	0.0
28	0.0	0.0	0.0	0.00404996216224867	0.0
29	0.0	0.0	0.0	0.006205587184090704	0.0
30	0.0	0.0	0.0	0.010255549346339373	0.0
31	0.0	0.0	0.0	0.018518778596733836	0.0
32	0.0	0.0	0.0	0.02838239612092011	0.0
33	0.0	0.0	0.0	0.03991172388925705	0.0
34	0.0	0.0	0.0	0.057254707019531594	0.0
35	0.0	0.0	0.0	0.0763940443346745	0.0
36	0.0	0.0	0.0	0.10105308814514019	0.0
37	0.0	0.0	0.0	0.13524913962799792	0.0
38	0.0	0.0	0.0	0.1715028331771594	0.0
39	0.0	0.0	0.0	0.21154520100713414	0.0
40	0.0	0.0	0.0	0.24943194381526684	0.0
41	0.0	0.0	0.0	0.28411791007581594	0.0
42	0.0	0.0	0.0	0.32252722864681943	0.0
43	0.0	0.0	0.0	0.3642679677061243	0.0
44	0.0	0.0	0.0	0.4135207333566968	0.0
45	0.0	0.0	0.0	0.4680972395915156	0.0
46	0.0	0.0	0.0	0.525645895477662	0.0
47	0.0	0.0	0.0	0.5848929225931385	0.0
48	0.0	0.0	0.0	0.6460016258638422	0.0
49	0.0	0.0	0.0	0.709037327260132	0.0
50	0.0	0.0	0.0	0.7725629434341131	0.0
51	0.0	0.0	0.0	0.8353373569489675	0.0
52	0.0	0.0	0.0	0.8966420261307478	0.0
53	0.0	0.0	0.0	0.961016827919394	0.0
54	0.0	0.0	0.0	1.0310093191589011	0.0
55	0.0	0.0	0.0	1.1095589885154178	0.0
56	0.0	0.0	0.0	1.1934323984561805	3.2660985179424757E-5
57	0.0	0.0	0.0	1.2825968879960101	3.2660985179424757E-5
58	0.0	0.0	0.0	1.3760726275795239	3.2660985179424757E-5
59	0.0	0.0	0.0	1.4718346361255972	3.2660985179424757E-5
60	0.0	0.0	0.0	1.5702748454563833	3.2660985179424757E-5
61	0.0	0.0	0.0	1.6687803767575284	3.2660985179424757E-5
62	0.0	0.0	0.0	1.7699314478582069	3.2660985179424757E-5
63	0.0	0.0	0.0	1.8698740625072467	3.2660985179424757E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4575	0.0	36.140636	1
GTATCAA	8945	0.0	34.074677	1
TATCAAC	11700	0.0	25.618769	2
TCAACGC	11590	0.0	25.506874	4
ATCAACG	11585	0.0	25.487688	3
CAACGCA	11875	0.0	24.835394	5
AACGCAG	12260	0.0	24.039358	6
GTGGTAT	2245	0.0	21.37223	1
GTACATG	13975	0.0	21.143326	1
ACGCAGA	14245	0.0	20.620659	7
TACATGG	14110	0.0	20.436361	2
CGCAGAG	14345	0.0	20.358404	8
AGAGTAC	13830	0.0	19.79614	11
TGGTATC	2320	0.0	19.476353	2
ACATGGG	14275	0.0	19.307383	3
GAGTACT	7645	0.0	19.16843	12
CAGAGTA	14950	0.0	18.704874	10
GCAGAGT	15290	0.0	18.491594	9
AGTACTT	8115	0.0	18.102213	13
GTACTTT	8130	0.0	17.812868	14
>>END_MODULE