FastQCFastQC Report
Mon 6 Feb 2017
SRR2077534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3061757
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58670.19162200004768504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38670.12630002968883552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37310.12185813570443377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57550.040.579341
GTATCAA115500.033.9086881
TATCAAC155750.025.0857242
ATCAACG156950.024.7429543
TCAACGC158350.024.4586834
GTGGTAT25500.024.0148371
CAACGCA161600.023.9081925
TGGTATC25300.023.2329652
AACGCAG166200.023.1391946
GCCGTAA3200.021.55571663
GTACATG128000.021.2180881
TACATGG133350.020.1467152
ACGCAGA193400.019.65257
CGCAGAG194150.019.5413428
ACATGGG133050.019.3370933
AGAGTAC190900.019.22363911
GAGTACT134550.018.9356812
AGTACTT139000.018.64533213
CAGAGTA202750.018.2703610
TGCGCGA950.00785823718.14951554
Produced by FastQC (version 0.11.5)