##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077534_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3061757 Sequences flagged as poor quality 0 Sequence length 75 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.859688407669193 31.0 31.0 34.0 27.0 34.0 2 30.93305608511714 33.0 31.0 34.0 27.0 34.0 3 30.995916070413166 33.0 31.0 34.0 27.0 34.0 4 34.358215233932675 37.0 35.0 37.0 31.0 37.0 5 34.074830889583986 37.0 35.0 37.0 30.0 37.0 6 34.04548891371849 37.0 35.0 37.0 30.0 37.0 7 33.88214479463916 37.0 35.0 37.0 29.0 37.0 8 33.83333197245895 37.0 35.0 37.0 29.0 37.0 9 35.26833481559771 39.0 35.0 39.0 28.0 39.0 10 35.17853115057792 39.0 35.0 39.0 28.0 39.0 11 35.16087592842933 39.0 35.0 39.0 27.0 39.0 12 35.058623855518256 39.0 35.0 39.0 27.0 39.0 13 34.983591121045855 39.0 35.0 39.0 27.0 39.0 14 36.21000915487414 40.0 36.0 41.0 27.0 41.0 15 36.266067490006556 40.0 36.0 41.0 27.0 41.0 16 36.25326177093741 40.0 36.0 41.0 27.0 41.0 17 36.27786725073218 40.0 37.0 41.0 27.0 41.0 18 36.29128111734537 40.0 37.0 41.0 27.0 41.0 19 36.355129750662776 40.0 37.0 41.0 27.0 41.0 20 36.33034790154803 40.0 37.0 41.0 27.0 41.0 21 36.31555835423909 40.0 37.0 41.0 27.0 41.0 22 36.29710097829449 40.0 37.0 41.0 27.0 41.0 23 36.272225392152286 40.0 37.0 41.0 27.0 41.0 24 36.26178661467909 40.0 36.0 41.0 27.0 41.0 25 36.24083230641752 40.0 36.0 41.0 27.0 41.0 26 36.167578942417705 40.0 36.0 41.0 27.0 41.0 27 36.123243941305596 40.0 36.0 41.0 27.0 41.0 28 36.10091460556798 40.0 36.0 41.0 27.0 41.0 29 36.052571448354655 40.0 36.0 41.0 27.0 41.0 30 35.96187842470843 40.0 36.0 41.0 27.0 41.0 31 35.85488103726063 40.0 36.0 41.0 27.0 41.0 32 35.76693186297933 40.0 35.0 41.0 27.0 41.0 33 35.69229497964731 40.0 35.0 41.0 27.0 41.0 34 35.620946730913005 40.0 35.0 41.0 26.0 41.0 35 35.536565116042844 40.0 35.0 41.0 26.0 41.0 36 35.475268938717214 40.0 35.0 41.0 25.0 41.0 37 35.411709681728496 40.0 35.0 41.0 25.0 41.0 38 35.34724898154883 40.0 35.0 41.0 25.0 41.0 39 35.2766427903978 40.0 35.0 41.0 25.0 41.0 40 35.208259505898084 40.0 35.0 41.0 24.0 41.0 41 35.15393906178707 40.0 35.0 41.0 24.0 41.0 42 35.07961670374233 40.0 35.0 41.0 24.0 41.0 43 34.97120705529537 40.0 35.0 41.0 24.0 41.0 44 34.833679811951114 40.0 35.0 41.0 23.0 41.0 45 34.723685125893404 40.0 35.0 41.0 22.0 41.0 46 34.56575913764548 39.0 35.0 41.0 20.0 41.0 47 34.38899984551354 39.0 34.0 41.0 18.0 41.0 48 34.26856474893337 39.0 34.0 41.0 15.0 41.0 49 34.17241538110307 39.0 34.0 41.0 15.0 41.0 50 34.169432453326635 39.0 34.0 41.0 14.0 41.0 51 34.09775432864202 39.0 34.0 41.0 13.0 41.0 52 34.038800597173456 39.0 34.0 41.0 11.0 41.0 53 33.952001416180316 39.0 34.0 41.0 9.0 41.0 54 33.926505924539406 39.0 34.0 41.0 9.0 41.0 55 33.85603233698821 39.0 34.0 41.0 8.0 41.0 56 33.74344632836636 39.0 34.0 41.0 7.0 41.0 57 33.67869396558904 39.0 34.0 41.0 7.0 41.0 58 33.58891642935739 39.0 33.0 41.0 2.0 41.0 59 33.50853382551261 39.0 33.0 41.0 2.0 41.0 60 33.48300534627666 38.0 34.0 41.0 2.0 41.0 61 33.38142739609969 38.0 33.0 41.0 2.0 41.0 62 33.2477182219229 38.0 33.0 41.0 2.0 41.0 63 33.118832422037414 38.0 33.0 41.0 2.0 41.0 64 32.983275289319174 37.0 33.0 40.0 2.0 41.0 65 32.85899011580606 37.0 33.0 40.0 2.0 41.0 66 32.67570613866483 37.0 33.0 40.0 2.0 41.0 67 32.49380502763609 37.0 33.0 40.0 2.0 41.0 68 32.28626275697255 36.0 33.0 40.0 2.0 41.0 69 32.1232899932947 36.0 32.0 39.0 2.0 41.0 70 31.93947070260638 36.0 32.0 39.0 2.0 41.0 71 31.761537574667095 36.0 32.0 39.0 2.0 41.0 72 31.5606117663812 36.0 32.0 39.0 2.0 41.0 73 31.358406953915676 35.0 32.0 39.0 2.0 41.0 74 31.092576582661525 35.0 32.0 38.0 2.0 40.0 75 30.873687232526944 35.0 32.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5432230310733708 1101 2 0.5720193940982803 1101 3 0.5679306861575348 1101 4 0.5440582176528181 1101 5 0.5738200256510595 1101 6 0.5473802993697277 1101 7 0.5542408476658878 1101 8 0.6881394370280418 1101 9 0.7754617248671991 1101 10 0.7893628834025463 1101 11 0.8585996292653277 1101 12 0.8627642855545758 1101 13 0.8297070948517486 1101 14 0.946606545061293 1101 15 0.8854098368544641 1101 16 0.912930682517576 1101 17 0.9463856963522588 1101 18 0.8455801068427604 1101 19 0.9271586485224148 1101 20 1.034319847334359 1101 21 0.8622983086091196 1101 22 0.8711956426129817 1101 23 0.8188608084951667 1101 24 0.9402468421985901 1101 25 0.9561372358429452 1101 26 0.9893447654076084 1101 27 0.9587464239480994 1101 28 0.9168697839395037 1101 29 0.9530808190448496 1101 30 0.9705692442556284 1101 31 0.9720635445741976 1101 32 0.8587244756919219 1101 33 0.9523179188907847 1101 34 0.9747757795677643 1101 35 1.0928345093625538 1101 36 0.9201054076324056 1101 37 1.0162587918899106 1101 38 1.0638719092332565 1101 39 0.9586073944634137 1101 40 1.0259850836422473 1101 41 0.9506640702186147 1101 42 0.9939126391768198 1101 43 1.0268028312928195 1101 44 1.1065538815637623 1101 45 1.0496130685826444 1101 46 1.0453299353834424 1101 47 1.0530529379039066 1101 48 1.118961717263609 1101 49 1.1596384673821447 1101 50 0.9697248805278988 1101 51 0.9647652797947543 1101 52 0.976411334892866 1101 53 0.951859170630847 1101 54 1.0148196017582052 1101 55 0.9935694187415649 1101 56 1.065903435671828 1101 57 1.0715318587414657 1101 58 1.0327380634934258 1101 59 0.9903953267894181 1101 60 0.9901773531209273 1101 61 0.9778258167546454 1101 62 0.9743870324538477 1101 63 0.9919050827640277 1101 64 1.0715867715871 1101 65 0.9931589178964586 1101 66 1.0579893287913862 1101 67 1.091504571568663 1101 68 1.0811727270674183 1101 69 1.0742007706915118 1101 70 1.042908814808186 1101 71 0.9784694034618724 1101 72 0.933383371244993 1101 73 0.8974024772977494 1101 74 1.0026104923457204 1101 75 1.1061492281859238 1102 1 0.7890561906265887 1102 2 0.8073219223549266 1102 3 0.866063267222632 1102 4 0.7992952478142428 1102 5 0.9567725734746091 1102 6 0.9383682548548009 1102 7 1.0002075812711695 1102 8 0.9939171969594653 1102 9 1.2475005782556465 1102 10 1.1937670335379167 1102 11 1.275805568906982 1102 12 1.2706431136347192 1102 13 1.2054525006231458 1102 14 1.5631222300847583 1102 15 1.4046070304017348 1102 16 1.4183558388974973 1102 17 1.438341858506412 1102 18 1.464413981630564 1102 19 1.438388652347058 1102 20 1.4101950335748725 1102 21 1.4551734274081625 1102 22 1.3978533274358966 1102 23 1.478032671557024 1102 24 1.5156415301176054 1102 25 1.531949106962287 1102 26 1.5423669643013866 1102 27 1.5606478298366255 1102 28 1.5197863614976441 1102 29 1.5528255397528454 1102 30 1.5670649053728667 1102 31 1.5999834942060644 1102 32 1.62145982203495 1102 33 1.6266843018004025 1102 34 1.5944028681459343 1102 35 1.628519923280173 1102 36 1.6406009826730212 1102 37 1.6428863720809304 1102 38 1.627180485925308 1102 39 1.6642496502742503 1102 40 1.6691594517999278 1102 41 1.6806847552287678 1102 42 1.6780203219143743 1102 43 1.6873312884892258 1102 44 1.740227321070087 1102 45 1.7122474080067107 1102 46 1.6660802350986756 1102 47 1.6835917908486593 1102 48 1.773806011759234 1102 49 1.7674721717914892 1102 50 1.7812703285389517 1102 51 1.7479127645383414 1102 52 1.6520225010713219 1102 53 1.7894129574252773 1102 54 1.7548354904371735 1102 55 1.7428362145527387 1102 56 1.7677836108066955 1102 57 1.7928011250403415 1102 58 1.7572956562942252 1102 59 1.7991818860584914 1102 60 1.717439149389108 1102 61 1.7435218216459276 1102 62 1.8150813956776588 1102 63 1.817243088785844 1102 64 1.8340023255433806 1102 65 1.7983381347412077 1102 66 1.9107601528995772 1102 67 1.7833949782851306 1102 68 1.7835279874580579 1102 69 1.7524900733200468 1102 70 1.8038916811240995 1102 71 1.816807086474519 1102 72 1.771426441500374 1102 73 1.7317981617532858 1102 74 1.8036660179765853 1102 75 1.8188046208860946 1103 1 0.7465780390219621 1103 2 0.7370286604824621 1103 3 0.7447686475769153 1103 4 0.7717610723770534 1103 5 0.8059683981754588 1103 6 0.9194123431661154 1103 7 0.8803542885455471 1103 8 0.8327601051876314 1103 9 0.9766915319554101 1103 10 1.010871400121637 1103 11 0.9471773319082004 1103 12 1.0410485612083988 1103 13 1.0679588743353818 1103 14 1.2392120041615016 1103 15 1.1725154886263596 1103 16 1.1484253609872468 1103 17 1.154155911752369 1103 18 1.166639269927316 1103 19 1.1813551380191143 1103 20 1.1995509550515777 1103 21 1.2194566080442897 1103 22 1.2020505345891763 1103 23 1.2048311041362254 1103 24 1.2178150548777253 1103 25 1.2985835547488804 1103 26 1.216692804366076 1103 27 1.2929142696221376 1103 28 1.2127470224646757 1103 29 1.1884070523691364 1103 30 1.1926897801469423 1103 31 1.29310190324383 1103 32 1.2904865922537923 1103 33 1.1748913174641515 1103 34 1.3151658292126385 1103 35 1.3565469817016478 1103 36 1.3155779975713031 1103 37 1.3715011296456225 1103 38 1.345481175812992 1103 39 1.4171533755338928 1103 40 1.3921740744238775 1103 41 1.2340363344244736 1103 42 1.273949135443921 1103 43 1.3347241421513019 1103 44 1.456299616848817 1103 45 1.3948366954016294 1103 46 1.3492094318666261 1103 47 1.403362565176792 1103 48 1.4335698278131659 1103 49 1.433164376809394 1103 50 1.311594976891989 1103 51 1.3276552770211936 1103 52 1.3815829104780235 1103 53 1.4357670371658031 1103 54 1.4587647402444475 1103 55 1.368250793423428 1103 56 1.479559899049491 1103 57 1.3864071675077696 1103 58 1.4141801125512146 1103 59 1.2660768560649203 1103 60 1.3048944288306359 1103 61 1.3821752648727283 1103 62 1.4043721369273214 1103 63 1.4787784203799177 1103 64 1.5683136341716022 1103 65 1.4430656891305702 1103 66 1.5381373541047623 1103 67 1.463815112063024 1103 68 1.426608363368004 1103 69 1.4575369449410331 1103 70 1.439229225394996 1103 71 1.4258659563604397 1103 72 1.4120455919593056 1103 73 1.428139452240213 1103 74 1.3675050465538199 1103 75 1.4082024446842674 1104 1 0.32023159178951843 1104 2 0.39751889287434494 1104 3 0.3639385331715026 1104 4 0.3879840693704537 1104 5 0.4078797635730069 1104 6 0.4906091228041731 1104 7 0.42292425741329964 1104 8 0.49411060040711874 1104 9 0.5321575985916311 1104 10 0.617657088549997 1104 11 0.5488376186794639 1104 12 0.607766647778412 1104 13 0.5268332696168798 1104 14 0.5927015298857157 1104 15 0.5523845589923866 1104 16 0.5722132045945898 1104 17 0.6662058620587885 1104 18 0.6338248923807086 1104 19 0.6350576133945509 1104 20 0.6335418345767607 1104 21 0.6371917329347951 1104 22 0.6360211279606744 1104 23 0.7439991949558333 1104 24 0.7652196238891449 1104 25 0.7874170014798096 1104 26 0.774262085453266 1104 27 0.7518065526943332 1104 28 0.7383092999321903 1104 29 0.725195967095388 1104 30 0.7128213143682558 1104 31 0.6936362185469207 1104 32 0.764007090837481 1104 33 0.7819204897026424 1104 34 0.7030588228498331 1104 35 0.8071816510081149 1104 36 0.8074073494471818 1104 37 0.8194298958662429 1104 38 0.8057981864373858 1104 39 0.7339334073231925 1104 40 0.7542698759345612 1104 41 0.6980536194265952 1104 42 0.7534130773303858 1104 43 0.7902811017485263 1104 44 0.7790928942972997 1104 45 0.7233766004894164 1104 46 0.786244383660275 1104 47 0.8156226770374388 1104 48 0.8502044038832679 1104 49 0.8150616128509327 1104 50 0.727420605109451 1104 51 0.7193915973957203 1104 52 0.7601811982167277 1104 53 0.7162167162373478 1104 54 0.812569250975649 1104 55 0.7790548491152123 1104 56 0.9223193935565135 1104 57 0.8312861153819 1104 58 0.8250768050137722 1104 59 0.8066404943536085 1104 60 0.8303402357950915 1104 61 0.8440124878761637 1104 62 0.8201773290934042 1104 63 0.7361933718048235 1104 64 0.829880551672062 1104 65 0.8669054473036297 1104 66 0.7949554129387053 1104 67 0.8622278254654887 1104 68 0.8911014236297206 1104 69 0.831444084622845 1104 70 0.870745044272816 1104 71 0.898688831107286 1104 72 0.9203934191498533 1104 73 0.9675185490376954 1104 74 0.9765895659151305 1104 75 0.9797705708597455 1105 1 0.39724324804328504 1105 2 0.4406854161838787 1105 3 0.4794859644961491 1105 4 0.37258211839835553 1105 5 0.39642301349617526 1105 6 0.4345679329217731 1105 7 0.5261819858044987 1105 8 0.6059197974938968 1105 9 0.7335826453124241 1105 10 0.7466966639118411 1105 11 0.6457697444062447 1105 12 0.7121994847451134 1105 13 0.6668721198348635 1105 14 0.7001408974252712 1105 15 0.6988850090284018 1105 16 0.7613458864313642 1105 17 0.6659570172146161 1105 18 0.7732734075232912 1105 19 0.8188675824469982 1105 20 0.7523351818869131 1105 21 0.7396727897101627 1105 22 0.772063915013419 1105 23 0.707247497189897 1105 24 0.8040996937463305 1105 25 0.7130848361565185 1105 26 0.7419580317541161 1105 27 0.7718868342716618 1105 28 0.7051853654551508 1105 29 0.7441530956683167 1105 30 0.8590146893912234 1105 31 0.8532398826268235 1105 32 0.7687482624902771 1105 33 0.7576628946466428 1105 34 0.8100272152764916 1105 35 0.7832793788193868 1105 36 0.8432419577043149 1105 37 0.8529558324153186 1105 38 0.9132144520487202 1105 39 0.88475581933087 1105 40 0.872042175058894 1105 41 0.832190259726481 1105 42 0.8783746870118989 1105 43 0.8988402948542671 1105 44 0.8719926402473916 1105 45 0.908049992225628 1105 46 0.9046236075162994 1105 47 0.9116911424062977 1105 48 0.8547303853527666 1105 49 0.7974866070457409 1105 50 0.8137262716654448 1105 51 0.794936376276759 1105 52 0.8047354534041418 1105 53 0.8130982649031537 1105 54 0.7961520035323701 1105 55 0.8406730155969342 1105 56 0.9820009434582246 1105 57 0.9063203226351959 1105 58 0.7936359363242289 1105 59 0.8526275444393931 1105 60 0.8841216673113053 1105 61 0.8974494344667434 1105 62 0.8913846971824668 1105 63 0.9148995735079524 1105 64 0.9025769959002758 1105 65 0.9552271136086361 1105 66 0.8407484733761521 1105 67 0.8857055640719267 1105 68 1.0129978922908336 1105 69 0.9548579126480021 1105 70 0.9843348961566605 1105 71 0.9049573444387846 1105 72 0.911189249679623 1105 73 0.8373255409836311 1105 74 0.8714895775849278 1105 75 1.021825777337984 1106 1 0.30317220206818973 1106 2 0.29618292493387344 1106 3 0.30883787117026884 1106 4 0.32500900382479614 1106 5 0.24982977831498943 1106 6 0.3242206588400691 1106 7 0.332329447915491 1106 8 0.4011302991416841 1106 9 0.3944036298234934 1106 10 0.4650844462435302 1106 11 0.3918800610634108 1106 12 0.3665777956306471 1106 13 0.3549995248740885 1106 14 0.3478658534796395 1106 15 0.36481027060735016 1106 16 0.4960090221006652 1106 17 0.5540024702114366 1106 18 0.4873331551662474 1106 19 0.4682929973407184 1106 20 0.5714674847116825 1106 21 0.5463658774697464 1106 22 0.586612658522867 1106 23 0.589115101363376 1106 24 0.595121202934628 1106 25 0.6341912163532228 1106 26 0.672294790191728 1106 27 0.6661529537319524 1106 28 0.6544022630454833 1106 29 0.593705029782555 1106 30 0.4804369993154296 1106 31 0.6185181834755369 1106 32 0.6432037648332738 1106 33 0.646143798439553 1106 34 0.5168615552080382 1106 35 0.5846995892980118 1106 36 0.5878644304276364 1106 37 0.579931083819325 1106 38 0.521535623023702 1106 39 0.5936680118610838 1106 40 0.6280506870215916 1106 41 0.5960479817727773 1106 42 0.5626603368409562 1106 43 0.6677844302648239 1106 44 0.6739491301585403 1106 45 0.7016179539494161 1106 46 0.5564633848277367 1106 47 0.573810099939486 1106 48 0.545118285548071 1106 49 0.6230439039543043 1106 50 0.5847198066621857 1106 51 0.5284497892478299 1106 52 0.5250983720386557 1106 53 0.5181495033458461 1106 54 0.5788981520165777 1106 55 0.5842077360990316 1106 56 0.6012825094972953 1106 57 0.57830036685246 1106 58 0.583099620059329 1106 59 0.5819930790935999 1106 60 0.48812645183728876 1106 61 0.5400604449538378 1106 62 0.5417811516976982 1106 63 0.5462990381493285 1106 64 0.6010234061706115 1106 65 0.6203278363519829 1106 66 0.6519548166483986 1106 67 0.6537106579898833 1106 68 0.6832420265043382 1106 69 0.6308578426664866 1106 70 0.626847103788144 1106 71 0.6503927537984424 1106 72 0.6421664771407407 1106 73 0.5782034499358311 1106 74 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-2.0264548797648274 2216 44 -2.0929333475474934 2216 45 -2.141684482274023 2216 46 -1.9679709168233614 2216 47 -2.313548841668492 2216 48 -2.318497145038897 2216 49 -2.167955896835501 2216 50 -2.1505620757569304 2216 51 -2.175585974840427 2216 52 -2.193844759311169 2216 53 -2.2308755385468864 2216 54 -2.2616141697195964 2216 55 -2.2934707620905 2216 56 -2.3899346647647484 2216 57 -2.2878713992044624 2216 58 -2.14922457694783 2216 59 -2.255259226471395 2216 60 -2.2472099204279665 2216 61 -2.2358702767729604 2216 62 -2.263056140449901 2216 63 -2.250000925524109 2216 64 -2.239099869782372 2216 65 -2.1478717722585827 2216 66 -2.149268568284281 2216 67 -2.167938802384864 2216 68 -2.3153927977805537 2216 69 -2.4103798385269 2216 70 -2.227836093418226 2216 71 -2.167285392843695 2216 72 -2.3458082583198454 2216 73 -2.30823678114799 2216 74 -2.3077684677008072 2216 75 -2.3413977296208692 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68446.0 3 27393.0 4 15594.0 5 9507.0 6 7031.0 7 6304.0 8 6096.0 9 6403.0 10 7053.0 11 7780.0 12 8236.0 13 8552.0 14 9100.0 15 9739.0 16 10451.0 17 11375.0 18 12167.0 19 13525.0 20 16148.0 21 18653.0 22 24360.0 23 30251.0 24 31016.0 25 31280.0 26 30456.0 27 30394.0 28 34339.0 29 41225.0 30 47003.0 31 51478.0 32 61790.0 33 77063.0 34 99233.0 35 136654.0 36 187180.0 37 277917.0 38 481390.0 39 852254.0 40 256921.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.67631699624871 19.162106252661808 14.477194007741836 24.684382743347648 2 14.761016087098405 20.551907654103374 39.1833397413429 25.503736517455323 3 17.952688472673685 25.396353094265077 29.631990969616524 27.018967463444714 4 11.595189345125565 16.814167686058 37.62211435935343 33.96852860946301 5 12.663732330476044 38.71970267369399 34.470582042791 14.14598295303896 6 30.96574483502239 37.926563531079424 17.392061764997155 13.715629868901036 7 27.333209402981318 33.23060617950713 21.2321443098163 18.204040107695256 8 26.16529199394571 35.18184262444826 19.619787315855003 19.03307806575103 9 26.178056493120945 16.027875019892292 19.82154288352742 37.972525603459346 10 14.140731026650801 30.52675632815241 33.3210164771439 22.011496168052886 11 33.65373056710474 24.28739517538931 23.136121077815204 18.922753179690748 12 22.887364583874866 26.5432116930085 30.163485145873242 20.405938577243386 13 27.59774191407727 23.089775227676817 26.2647795018782 23.04770335636771 14 21.30961281569337 22.35073758728051 28.469947533663937 27.869702063362183 15 23.11180177181257 29.914747976175036 24.96750413813853 22.00594611387386 16 22.03695614459532 29.389011824829296 26.59150675828217 21.982525272293216 17 20.810959584327676 28.88909578855725 27.815972545502955 22.48397208161212 18 20.57173358909519 27.91726461127258 29.835445862234216 21.675555937398016 19 21.582491758467906 27.50692184166067 30.338343801570822 20.572242598300605 20 21.864783925533555 26.782447755109505 30.362271310192355 20.990497009164578 21 22.395240333929348 26.571840123467478 30.182133479609014 20.85078606299416 22 21.797971344282494 26.613995182996163 30.148087098067155 21.439946374654184 23 21.538355501229205 26.70407737280545 29.931330589867034 21.826236536098314 24 21.093822737784464 26.78632156417945 30.398803559918864 21.72105213811722 25 21.48573485605498 26.6811878587764 30.34838774588219 21.484689539286432 26 21.21814866520972 26.85488458194628 30.44878318761163 21.478183565232364 27 21.318164265285976 27.171909497863034 30.10057898419996 21.409347252651038 28 21.160618978491406 26.8767045319025 30.34567506733177 21.617001422274328 29 21.260740004398446 27.086035388218498 30.28513766060785 21.368086946775197 30 21.432697485009903 27.0205261039348 30.231372815523834 21.315403595531464 31 21.607961240469617 26.685038961089873 30.15287486879849 21.55412492964202 32 21.169622085711616 27.15094032695038 30.08579131908399 21.59364626825401 33 21.330541500207428 26.814010995292865 30.21193541327079 21.64351209122892 34 21.492684552139103 26.892429107610088 30.246918822864572 21.367967517386237 35 21.67988858055177 26.92050912207537 29.958674986542917 21.44092731082995 36 21.47621674749409 26.964380731944647 29.934531258114998 21.624871262446263 37 21.65531342937448 26.63947986875865 30.04492844275829 21.660278259108583 38 21.253968808699256 26.903468577944125 30.349444603579663 21.493118009776964 39 21.545024424261925 27.19247559615287 29.830818502664542 21.431681476920662 40 21.626502777310492 26.7845751066092 30.289718969822776 21.299203146257533 41 21.485792979637807 27.007204852406197 29.805818170652792 21.701183997303204 42 21.507159482795227 26.960634766754183 29.711020684402 21.82118506604859 43 21.224946568685574 26.769488669752178 29.87965990800962 22.12590485355263 44 21.34411998544475 27.181082876739627 29.689903894836352 21.78489324297928 45 21.747928045513426 26.935506313361437 29.435733318700468 21.880832322424666 46 21.787708037474506 26.89410616557817 29.229665271802535 22.08852052514479 47 21.588081549356776 27.095942456344808 29.105182051511523 22.210793942786893 48 21.31814937785087 27.346112200786198 29.21672412638634 22.119014294976587 49 21.69121333473124 27.269517548801836 28.858722828580934 22.18054628788599 50 21.850735934129283 27.28843424767821 28.82144391043699 22.03938590775552 51 21.728988293424106 27.60850351667604 28.472762499763206 22.18974569013665 52 21.784120486941163 27.56644063334621 28.573350975277002 22.076087904435624 53 21.834750439045294 27.476347731057686 28.558765440889005 22.13013638900801 54 21.569771866284622 27.53108754221841 28.754045471276786 22.145095120220187 55 21.71611567278737 27.400595739677435 28.566076805999202 22.31721178153599 56 21.588439634884264 27.805075624817288 28.51198515757824 22.094499582720207 57 21.795737753750238 27.405972945501674 28.337228808311277 22.461060492436815 58 21.537260614453523 27.20105691362279 28.923832650988246 22.337849820935446 59 21.748750540172328 27.49833987855064 28.600079110952294 22.152830470324737 60 21.622954567723813 27.66325897377274 28.61224156514355 22.101544893359897 61 21.898340669088835 27.455420424363332 28.63998489735821 22.006254009189618 62 21.55282530560135 27.624996161402727 28.732472311462804 22.089706221533127 63 21.52321504106667 27.292637396557552 28.898982029962582 22.285165532413192 64 21.695243340248442 27.181631488466273 28.736109763694085 22.3870154075912 65 21.38671865429739 27.32391863299884 28.747491621861855 22.541871090841916 66 21.49110757509879 27.544842694092942 28.51393946362882 22.45011026717945 67 21.57846837529555 27.50416007076857 28.351592782808044 22.565778771127835 68 21.603842123281407 27.36709237873478 28.696429924762125 22.332635573221694 69 21.624359148562558 27.558898232007817 28.16669453924023 22.650048080189393 70 21.532948050895957 27.190929094268323 28.56508206960171 22.711040785234005 71 21.62735003204255 27.22320102797468 28.517935662738598 22.63151327724417 72 21.54457721203735 27.578637277259304 28.14471526071633 22.732070249987014 73 21.591389757453953 27.323951850737675 28.44008109583242 22.644577295975946 74 21.68525767046327 27.481038221483505 28.05474268234439 22.778961425708832 75 21.52472870685643 27.485910720263902 28.173954225150855 22.815406347728814 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2973.0 1 1924.0 2 1202.0 3 1562.5 4 1596.0 5 1235.5 6 1050.0 7 2130.5 8 3036.0 9 4896.0 10 7985.0 11 8746.5 12 8279.0 13 7155.5 14 5868.5 15 6201.5 16 6698.0 17 7274.5 18 8425.0 19 9964.0 20 10929.0 21 12100.5 22 15183.5 23 19092.5 24 21090.0 25 24261.0 26 31092.5 27 39109.0 28 43465.0 29 48447.5 30 58395.0 31 68407.5 32 73455.0 33 81458.0 34 95554.0 35 107720.5 36 113794.0 37 116465.5 38 121481.0 39 126172.0 40 128519.0 41 131477.0 42 135966.0 43 139314.5 44 141132.0 45 141630.5 46 142443.5 47 144015.5 48 145273.0 49 144422.5 50 139355.0 51 132050.5 52 128963.0 53 124240.5 54 114575.0 55 104191.0 56 98750.0 57 93139.0 58 80420.0 59 66741.0 60 60170.0 61 54087.0 62 41915.0 63 30239.0 64 24652.0 65 20492.0 66 13331.0 67 8647.5 68 6965.0 69 5764.5 70 3674.5 71 2269.0 72 1753.0 73 1666.5 74 1422.0 75 943.0 76 622.0 77 516.5 78 314.0 79 185.0 80 153.0 81 130.5 82 71.0 83 26.5 84 19.0 85 15.0 86 8.5 87 3.0 88 0.0 89 1.0 90 1.5 91 1.5 92 2.0 93 1.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07433640226837074 2 0.015807916826841583 3 0.003037471621686502 4 0.14295713213034214 5 0.098962785093657 6 0.07054772798755747 7 0.02932956469112343 8 0.025671534351027855 9 0.04984066338380217 10 0.02975415749845595 11 0.014795426286279414 12 0.10823850488461363 13 0.08838062589552338 14 0.04928542663575195 15 0.019531269137296 16 0.03246501926834821 17 0.02260140174416193 18 0.01881272746334866 19 0.014142206582690917 20 0.013227698997667027 21 0.028415057106099536 22 0.016918390322942022 23 0.013815596730896672 24 0.022078825981291135 25 0.015807916826841583 26 0.033118238971936706 27 0.028709005972714356 28 0.03808270871920927 29 0.05180032249456766 30 0.03491459315680506 31 0.020445776722319897 32 0.012247869442284282 33 0.014599460375202867 34 0.01394624067161437 35 0.0056503504360404825 36 0.009667651613109727 37 0.007250738709832296 38 0.002286268962559733 39 0.00823056826521504 40 0.011170056931363265 41 0.011366022842439816 42 0.0072833996950117195 43 0.011431344812798663 44 0.010418854272236497 45 0.005062452702810838 46 0.0016003882737918131 47 0.004931808762093138 48 0.006270909154449554 49 0.0012411174368181406 50 6.532197035884951E-5 51 0.0019269981255860605 52 0.003723352310454422 53 0.0 54 0.0 55 5.552367480502209E-4 56 0.008557178117009286 57 0.002776183740251104 58 3.919318221530971E-4 59 0.007642670531985393 60 0.001861676155227211 61 0.0013391003923564151 62 0.024267111988312592 63 0.008883787968803535 64 0.011235378901722116 65 0.016853068352583173 66 0.018159507759760163 67 0.01668976342668605 68 0.017408305100633396 69 0.006695501961782075 70 0.014795426286279414 71 0.006630179991423226 72 0.016918390322942022 73 0.006336231124808403 74 0.01956393012247543 75 0.0010451515257415922 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3061757.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.40461372348432 #Duplication Level Percentage of deduplicated Percentage of total 1 73.48134615432672 39.97724253407785 2 12.655681185689327 13.770548926299917 3 5.372436612302664 8.768560159386933 4 2.8345633202949463 6.168532900616149 5 1.632737831167532 4.441423550819457 6 1.046127664422572 3.414850289301652 7 0.7068050925524588 2.691742062667567 8 0.49067266256118114 2.1355885337051697 9 0.35482919978783006 1.7373910997042945 >10 1.3455550156463503 11.696270652153457 >50 0.05192116651637899 1.9557020632102393 >100 0.026297394350413245 2.4118786759790773 >500 6.643355411791534E-4 0.24452327223650716 >1k 3.019707004024784E-4 0.39297209552406775 >5k 6.0394140080495677E-5 0.1927731843177011 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5867 0.19162200004768504 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3867 0.12630002968883552 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3731 0.12185813570443377 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.2660985179424757E-5 0.0 8 0.0 0.0 0.0 1.6330492589712378E-4 0.0 9 0.0 0.0 0.0 2.6128788143539805E-4 0.0 10 0.0 0.0 0.0 2.6128788143539805E-4 0.0 11 0.0 0.0 0.0 2.6128788143539805E-4 0.0 12 0.0 0.0 0.0 2.939488666148228E-4 0.0 13 0.0 0.0 0.0 2.939488666148228E-4 0.0 14 0.0 0.0 0.0 3.2660985179424755E-4 0.0 15 0.0 0.0 0.0 3.2660985179424755E-4 0.0 16 0.0 0.0 0.0 3.9193182215309705E-4 0.0 17 0.0 0.0 0.0 4.5725379251194655E-4 0.0 18 0.0 0.0 0.0 4.899147776913713E-4 0.0 19 0.0 0.0 0.0 5.225757628707961E-4 0.0 20 0.0 0.0 0.0 5.225757628707961E-4 0.0 21 0.0 0.0 0.0 6.205587184090703E-4 0.0 22 0.0 0.0 0.0 7.512026591267694E-4 0.0 23 0.0 0.0 0.0 0.0010451515257415922 0.0 24 0.0 0.0 0.0 0.0014370833478946892 0.0 25 0.0 0.0 0.0 0.0015350663034329635 0.0 26 0.0 0.0 0.0 0.0020576420663037595 0.0 27 0.0 0.0 0.0 0.0026128788143539804 0.0 28 0.0 0.0 0.0 0.00398464019188982 0.0 29 0.0 0.0 0.0 0.0061729261989112785 0.0 30 0.0 0.0 0.0 0.010288210331518798 0.0 31 0.0 0.0 0.0 0.018649422537451533 0.0 32 0.0 0.0 0.0 0.028676344987534935 0.0 33 3.2660985179424757E-5 0.0 0.0 0.04030365571141015 0.0 34 3.2660985179424757E-5 0.0 0.0 0.05780994376758181 0.0 35 3.2660985179424757E-5 0.0 0.0 0.07701460305308357 0.0 36 3.2660985179424757E-5 0.0 0.0 0.10151034193765214 0.0 37 3.2660985179424757E-5 0.0 0.0 0.13531446159835675 0.0 38 3.2660985179424757E-5 0.0 0.0 0.17130686726608285 0.0 39 3.2660985179424757E-5 0.0 0.0 0.21079399834800738 0.0 40 3.2660985179424757E-5 0.0 0.0 0.24926863888936973 0.0 41 3.2660985179424757E-5 0.0 0.0 0.28428121500171305 0.0 42 3.2660985179424757E-5 0.0 0.0 0.32278851652825485 0.0 43 3.2660985179424757E-5 0.0 0.0 0.36433328967648315 0.0 44 3.2660985179424757E-5 0.0 0.0 0.41355339434187627 0.0 45 3.2660985179424757E-5 0.0 0.0 0.4680972395915156 0.0 46 3.2660985179424757E-5 0.0 0.0 0.5257438784332003 0.0 47 3.2660985179424757E-5 0.0 0.0 0.5855134813115476 0.0 48 3.2660985179424757E-5 0.0 0.0 0.6474060482265575 0.0 49 3.2660985179424757E-5 0.0 0.0 0.7101804617414119 0.0 50 3.2660985179424757E-5 0.0 0.0 0.7741306707227256 0.0 51 3.2660985179424757E-5 0.0 0.0 0.8375909649263479 0.0 52 3.2660985179424757E-5 0.0 0.0 0.8989282950933075 0.0 53 3.2660985179424757E-5 0.0 0.0 0.964707519244669 0.0 54 3.2660985179424757E-5 0.0 0.0 1.0353532301877648 0.0 55 3.2660985179424757E-5 0.0 0.0 1.1143274923516138 0.0 56 3.2660985179424757E-5 0.0 0.0 1.19914807086258 0.0 57 3.2660985179424757E-5 0.0 0.0 1.2898476267058425 0.0 58 3.2660985179424757E-5 0.0 0.0 1.383780620081868 0.0 59 3.2660985179424757E-5 0.0 0.0 1.4799998824204534 0.0 60 3.2660985179424757E-5 0.0 0.0 1.578701379632675 0.0 61 3.2660985179424757E-5 0.0 0.0 1.6779581135929469 0.0 62 3.2660985179424757E-5 0.0 0.0 1.7804156241008022 0.0 63 3.2660985179424757E-5 0.0 0.0 1.8816320171718395 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5755 0.0 40.57934 1 GTATCAA 11550 0.0 33.908688 1 TATCAAC 15575 0.0 25.085724 2 ATCAACG 15695 0.0 24.742954 3 TCAACGC 15835 0.0 24.458683 4 GTGGTAT 2550 0.0 24.014837 1 CAACGCA 16160 0.0 23.908192 5 TGGTATC 2530 0.0 23.232965 2 AACGCAG 16620 0.0 23.139194 6 GCCGTAA 320 0.0 21.555716 63 GTACATG 12800 0.0 21.218088 1 TACATGG 13335 0.0 20.146715 2 ACGCAGA 19340 0.0 19.6525 7 CGCAGAG 19415 0.0 19.541342 8 ACATGGG 13305 0.0 19.337093 3 AGAGTAC 19090 0.0 19.223639 11 GAGTACT 13455 0.0 18.93568 12 AGTACTT 13900 0.0 18.645332 13 CAGAGTA 20275 0.0 18.27036 10 TGCGCGA 95 0.007858237 18.149515 54 >>END_MODULE