FastQCFastQC Report
Mon 6 Feb 2017
SRR2077533_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077533_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5183639
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38150.034.7464451
GTATCAA76050.034.3613621
TATCAAC97750.026.1861112
TCAACGC96600.026.1070444
ATCAACG96950.026.0471213
CAACGCA98000.025.6297135
AACGCAG103850.024.3647986
GTACATG134000.020.6348531
ACGCAGA122600.020.5557697
TACATGG136250.019.7488572
CGCAGAG129950.019.156038
ACATGGG145600.018.4574873
AGAGTAC125550.018.40113611
TAGCGCG2251.1120028E-716.86366829
CAGAGTA139250.016.73868210
GAGTACT69700.016.62237512
GCAGAGT145200.016.4562999
CGAATCG2955.347829E-1016.37693637
CATGGGG111050.016.2169594
GTATAGG12650.016.1003441
Produced by FastQC (version 0.11.5)