FastQCFastQC Report
Mon 6 Feb 2017
SRR2077533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5183639
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91750.035.852761
GGTATCA43700.035.6187931
TATCAAC128000.025.413912
ATCAACG129350.024.9382443
TCAACGC129650.024.8805394
CAACGCA131000.024.5412045
AACGCAG137450.023.4859896
GTACATG128250.020.4706941
ACGCAGA162250.019.66157
TACATGG132350.019.0151312
CGAATCG5100.018.93532837
CGCAGAG169350.018.7560088
CGTATAC2405.9299055E-1018.7276293
AGAGTAC165300.018.15156611
GAGTACT110400.017.88933212
ACATGGG138950.017.6416153
CAGAGTA179150.016.9602810
AGTACTT117750.016.79404613
TATACCG2904.1654857E-1016.6704585
GCAGAGT185150.016.6521269
Produced by FastQC (version 0.11.5)