FastQCFastQC Report
Mon 6 Feb 2017
SRR2077532_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077532_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4822991
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33300.036.0816651
GTATCAA70700.034.91711
TCAACGC90700.026.472884
ATCAACG90950.026.3619043
TATCAAC93250.025.9312362
CAACGCA92050.025.8988535
AACGCAG95450.024.9932336
ACGCAGA112250.021.1312877
GTACATG118450.020.7537441
CGCAGAG115850.020.179148
TACATGG121050.019.6624832
AGAGTAC115300.019.02078211
CCGCGTA1108.2710123E-418.80832510
ACATGGG125200.018.4889763
GAGTACT66350.017.67026512
CAGAGTA124950.017.66181410
CGCACGA5500.017.56064235
GCAGAGT128900.017.5482359
GTGTAGC22200.017.1076851
GTATAGC13500.016.368081
Produced by FastQC (version 0.11.5)