FastQCFastQC Report
Mon 6 Feb 2017
SRR2077532_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077532_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4822991
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44700.037.9293941
GTATCAA86800.033.1658331
TCAACGC116800.024.6351644
ATCAACG116700.024.4490833
TATCAAC118100.024.2733632
CAACGCA119350.023.8133545
AACGCAG124000.023.0274476
GTACATG117950.020.0946271
ACGCAGA146400.019.3864027
TACATGG122600.019.0669632
GTGGTAT22700.019.0533541
CGCAGAG150950.018.7566918
ACATGGG124200.018.266773
AGAGTAC147500.018.25904711
GAGTACT99400.017.75047712
TAGATCG2751.9463187E-1017.5807955
CAGAGTA157250.017.36846210
TGGTATC23050.016.9524632
GCAGAGT162850.016.9408239
AGTACTT106900.016.65918213
Produced by FastQC (version 0.11.5)