FastQCFastQC Report
Mon 6 Feb 2017
SRR2077531_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077531_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5318052
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38300.035.9679151
GTATCAA75350.034.5943221
TATCAAC101350.025.6299632
TCAACGC100150.025.4916784
CAACGCA100850.025.3494285
ATCAACG101200.025.3287473
AACGCAG107050.023.829736
GTACATG145500.020.6204381
ACGCAGA124750.020.3410157
TACATGG148650.019.609592
CGCAGAG131600.019.0487778
AGAGTAC127450.018.69558311
ACATGGG154050.018.229223
GCACCGT13700.018.1169856
CGCCCAA9150.017.72626550
CGTGCCG9550.017.69217710
CGAATCG7700.017.4789137
AGCACCG15950.017.3043445
CAGAGTA139900.017.22852110
GAGTACT64300.016.78549612
Produced by FastQC (version 0.11.5)