FastQCFastQC Report
Mon 6 Feb 2017
SRR2077531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5318052
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45650.037.3564151
GTATCAA93750.037.191891
TATCAAC131050.026.4871082
ATCAACG134000.025.8300063
TCAACGC133850.025.7802354
CAACGCA134250.025.5693055
AACGCAG141050.024.2821586
GCGATAC6450.021.92912714
GTGGTAT21100.020.9839151
ACGCAGA165050.020.5626227
GTACATG139550.020.276011
CGCAGAG171900.019.7238868
TACATGG143300.019.445842
TGCGATA7350.019.2526213
GAGTACT110650.019.09753412
AGAGTAC168800.018.81883211
CGCACGA7800.018.57004535
ACATGGG146450.018.3453873
AGTACTT120750.017.72139413
GTACTTT119400.017.62473914
Produced by FastQC (version 0.11.5)