FastQCFastQC Report
Mon 6 Feb 2017
SRR2077530_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077530_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5210579
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34850.032.3930471
GTATCAA68900.032.368321
TCAACGC83950.025.8057024
CAACGCA86050.025.1370475
ATCAACG86500.025.0444743
TATCAAC87900.024.8798542
AACGCAG92050.023.4437246
GTACATG114650.019.6026041
ACGCAGA108600.019.5551157
CGCAGAG112900.018.7508748
CGTGCCG7600.018.14739810
TACATGG119350.017.9769272
AGAGTAC112450.017.66248511
GCACCGT11150.017.6228476
ACATGGG123700.017.0388343
GAGTACT62300.016.8815812
CAGAGTA124200.016.29612710
GTATAGA14150.016.1025371
CGCACGA7750.016.02439735
GCAGAGT131350.015.6451579
Produced by FastQC (version 0.11.5)