FastQCFastQC Report
Mon 6 Feb 2017
SRR2077530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5210579
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.038.553611
GTATCAA81400.034.5177151
TATCAAC111200.024.7548032
CGCGTAA700.001338050224.63155747
TCAACGC111800.024.5336424
ATCAACG113300.024.2696593
CAACGCA112800.024.2200785
AACGCAG119550.022.8433486
GCGATAC6650.020.75132614
ACGCAGA140900.019.1609087
CGCAGAG146200.018.3727198
GTACATG115100.017.88761
AGAGTAC144200.017.43073311
TGCGATA7750.017.36849613
GAGTACT93500.017.17081512
TACATGG117950.017.0931382
GTGGTAT22350.016.5659621
CGATACT8550.016.54103715
TAGCGCG1907.0335373E-616.34088529
GTACTTT98400.016.26785914
Produced by FastQC (version 0.11.5)