FastQCFastQC Report
Mon 6 Feb 2017
SRR2077529_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077529_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3953174
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31600.035.83551
GTATCAA61550.034.0476651
TCAACGC76900.026.7385734
ATCAACG77600.026.4967043
CAACGCA77600.026.453255
TATCAAC79700.026.0139882
AACGCAG83100.024.8923456
GTACATG95550.021.7155321
ACGCAGA95150.021.4879287
TACATGG97850.020.6951262
CGCAGAG98450.020.6296678
AGAGTAC96550.019.53687911
ACATGGG100900.018.9420013
GAGTACT57350.017.91855412
CAGAGTA108650.017.6780610
GCAGAGT113550.017.1577159
CGTGCCG5850.016.5048410
GTACTTT62850.016.3515414
CATGGGG75600.016.2916374
AGTACTT63300.016.28936413
Produced by FastQC (version 0.11.5)