FastQCFastQC Report
Mon 6 Feb 2017
SRR2077529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3953174
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38750.037.9434781
GTATCAA75750.033.15691
TCAACGC101050.024.7052574
TATCAAC101500.024.6613582
ATCAACG101000.024.6496453
CAACGCA101100.024.5552485
AACGCAG107200.023.2516616
GTACATG94000.020.6837081
TACATGG94950.019.9175932
ACGCAGA125500.019.5858467
CGAATCG5650.018.92278937
CGCAGAG129600.018.8867978
GTGGTAT20850.018.7496131
AGAGTAC126550.018.52427511
ACATGGG100500.018.0287153
TACCGAT6250.017.6756767
GAGTACT87950.017.54709612
GCGATAC4350.017.44699714
GTACCGA6800.017.2616216
CGCACGA5800.017.24415235
Produced by FastQC (version 0.11.5)