FastQCFastQC Report
Mon 6 Feb 2017
SRR2077528_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077528_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4437321
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30100.033.0310021
GTATCAA58600.032.813561
ATCAACG72550.025.6792433
TCAACGC72800.025.6393174
CAACGCA75350.024.7727495
TATCAAC76500.024.5774272
AACGCAG79150.023.5193566
ACGCAGA92600.019.769947
GTACATG100000.019.0216141
CGCAGAG96350.018.6447628
TACATGG102500.017.8382952
AGAGTAC95900.017.25909411
GTATAGC16650.017.2091561
TAGCCCT29650.017.10544
ACATGGG105550.016.898893
GTGGTAT19150.016.224431
CAGAGTA105350.015.84150210
GTGTAGC24300.015.7693021
GCAGAGT112800.015.2537629
GAGTACT53400.015.11002112
Produced by FastQC (version 0.11.5)