FastQCFastQC Report
Mon 6 Feb 2017
SRR2077528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4437321
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35950.036.3721051
GTATCAA70200.033.5571861
TCAACGC92800.024.9640984
ATCAACG92900.024.8627833
CAACGCA94450.024.4576595
TATCAAC95400.024.3905372
AACGCAG99000.023.3629386
ACGCAGA116400.019.6930397
GTACATG98850.019.4918941
TACATGG99900.018.9657382
CGCAGAG120650.018.9143478
GTATAGG9400.018.7679941
AGAGTAC121300.017.8751811
ACATGGG102350.017.3307973
CGAATCG1200.001481043917.24414337
GAGTACT80700.017.07068412
CAGAGTA130150.016.84539210
GCAGAGT136700.016.1885059
AGTACTT86300.016.11517713
TATCTCG5600.016.01187536
Produced by FastQC (version 0.11.5)