FastQCFastQC Report
Mon 6 Feb 2017
SRR2077527_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077527_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5138911
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96650.034.5836451
GGTATCA49850.033.5256731
TCAACGC121550.027.3361954
ATCAACG121700.027.2158573
TATCAAC123400.026.7552132
CAACGCA125500.026.421345
AACGCAG130850.025.3171126
ACGCAGA149550.021.9915647
CGCAGAG152450.021.4622488
AGAGTAC146200.020.63666211
GTACATG131100.020.2017941
GAGTACT91950.019.9871112
TACATGG130950.019.6273842
CAGAGTA163000.018.9743410
AGTACTT99100.018.58220313
GCAGAGT171100.018.5795129
GTACTTT97800.018.51221814
ACATGGG135250.018.0608123
TACTTTT113450.016.1404415
ACTTTTT116600.015.97073216
Produced by FastQC (version 0.11.5)