FastQCFastQC Report
Mon 6 Feb 2017
SRR2077527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5138911
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57100.035.500971
GTATCAA112250.035.223991
TATCAAC152050.025.8090462
ATCAACG151350.025.7717723
TCAACGC153100.025.4322644
CAACGCA157600.024.7585875
AACGCAG163150.023.8286766
ACGCAGA188700.020.4717987
CGCAGAG192900.019.9196598
AGAGTAC187700.019.31312211
GAGTACT133000.019.02741212
GTACATG122900.018.9708461
CAGAGTA203750.017.96121410
AGTACTT141300.017.92578913
GCAGAGT207900.017.8016099
TACATGG126300.017.7392062
GTACTTT142650.017.55680514
ACATGGG126050.016.9810853
TACTTTT157100.015.91858215
ACTTTTT168600.015.34397316
Produced by FastQC (version 0.11.5)